Smart Nanofibers with Natural Extracts Prevent Senescence Patterning in a Dynamic Cell Culture Model of Human Skin
Emanuela Bellu,
Giuseppe Garroni,
Sara Cruciani,
Francesca Balzano,
Diletta Serra,
Rosanna Satta,
Maria Antonia Montesu,
Angela Fadda,
Maurizio Mulas,
Giorgia Sarais,
Pasquale Bandiera,
Elena Torreggiani,
Fernanda Martini,
Mauro Tognon,
Carlo Ventura,
Jiří Beznoska,
Evzen Amler,
Margherita Maioli
Affiliations
Emanuela Bellu
Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43/B, 07100 Sassari, Italy
Giuseppe Garroni
Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43/B, 07100 Sassari, Italy
Sara Cruciani
Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43/B, 07100 Sassari, Italy
Francesca Balzano
Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43/B, 07100 Sassari, Italy
Diletta Serra
Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43/B, 07100 Sassari, Italy
Rosanna Satta
Department of Medical, Surgical and Experimental Sciences, University of Sassari, 07100 Sassari, Italy
Maria Antonia Montesu
Department of Medical, Surgical and Experimental Sciences, University of Sassari, 07100 Sassari, Italy
Angela Fadda
Istituto di Scienze delle Produzioni Alimentari (ISPA), Consiglio Nazionale delle Ricerche (CNR), Traversa la Crucca 3, 07100 Sassari, Italy
Maurizio Mulas
Department of Agriculture, University of Sassari, Via De Nicola 9, 07100 Sassari, Italy
Giorgia Sarais
Department of Life and Environmental Sciences, University of Cagliari, Via Ospedale 72, 09124 Cagliari, Italy
Pasquale Bandiera
Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43/B, 07100 Sassari, Italy
Elena Torreggiani
Department Medical Sciences, Section Experimental Medicine, University of Ferrara, 44121 Ferrara, Italy
Fernanda Martini
Department Medical Sciences, Section Experimental Medicine, University of Ferrara, 44121 Ferrara, Italy
Mauro Tognon
Department Medical Sciences, Section Experimental Medicine, University of Ferrara, 44121 Ferrara, Italy
Carlo Ventura
Laboratory of Molecular Biology and Stem Cell Engineering-Eldor Lab, National Institute of Biostructures and Biosystems, Innovation Accelerator, CNR, Via Piero Gobetti 101, 40129 Bologna, Italy
Jiří Beznoska
Institute of Biophysics, 2nd Faculty of Medicine, Charles University, V Uvalu 84, 150 06 Prague 5, Czech Republic
Evzen Amler
Institute of Biophysics, 2nd Faculty of Medicine, Charles University, V Uvalu 84, 150 06 Prague 5, Czech Republic
Margherita Maioli
Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43/B, 07100 Sassari, Italy
Natural cosmetic products have recently re-emerged as a novel tool able to counteract skin aging and skin related damages. In addition, recently achieved progress in nanomedicine opens a novel approach yielding from combination of modern nanotechnology with traditional treatment for innovative pharmacotherapeutics. In the present study, we investigated the antiaging effect of a pretreatment with Myrtus communis natural extract combined with a polycaprolactone nanofibrous scaffold (NanoPCL-M) on skin cell populations exposed to UV. We set up a novel model of skin on a bioreactor mimicking a crosstalk between keratinocytes, stem cells and fibroblasts, as in skin. Beta-galactosidase assay, indicating the amount of senescent cells, and viability assay, revealed that fibroblasts and stem cells pretreated with NanoPCL-M and then exposed to UV are superimposable to control cells, untreated and unexposed to UV damage. On the other hand, cells only exposed to UV stress, without NanoPCL-M pretreatment, exhibited a significantly higher yield of senescent elements. Keratinocyte-based 3D structures appeared disjointed after UV-stress, as compared to NanoPCL-M pretreated samples. Gene expression analysis performed on different senescence associated genes, revealed the activation of a molecular program of rejuvenation in stem cells pretreated with NanoPCL-M and then exposed to UV. Altogether, our results highlight a future translational application of NanoPCL-M to prevent skin aging.
