Frontiers in Bioscience-Landmark (Mar 2024)

Chitosan Membranes Stabilized with Varying Acyl Lengths Release Cis-2-Decenoic Acid and Bupivacaine at Controlled Rates and Inhibit Pathogenic Biofilm

  • Landon Reed Choi,
  • Zoe Harrison,
  • Emily C. Montgomery,
  • Joshua R. Bush,
  • Ezzuddin Abuhussein,
  • Joel D. Bumgardner,
  • Tomoko Fujiwara,
  • Jessica Amber Jennings

DOI
https://doi.org/10.31083/j.fbl2903108
Journal volume & issue
Vol. 29, no. 3
p. 108

Abstract

Read online

Background: Adherence of complex bacterial biofilm communities to burned tissue creates a challenge for treatment, with infection causing 51% of burn victim deaths. This study evaluated the release of therapeutics from wound care biomaterials and their antimicrobial activity against pathogens Staphylococcus aureus, Acinetobacter baumannii, and Pseudomonas aeruginosa. Methods: Electrospun chitosan membranes (ESCMs) were fabricated and acylated with chain lengths ranging from 6–10 carbons then loaded with 0.15 mg of anti-biofilm agent, cis-2-decenoic acid (C2DA), and 0.5 mg of local anesthetic, bupivacaine. Results: Combinations of therapeutics released from modified ESCMs at a cumulative amount of 45–70% of bupivacaine and less than 20% of C2DA. Results from bacterial studies suggest that this combination reduced biofilm 10-fold for S. aureus, 2-fold for Acinetobacter baumannii, and 2–3-fold for Pseudomonas aeruginosa by 24 hours. Additionally, dual loaded groups reduced planktonic Staphylococcus aureus ~4-fold by 24 hours as well as Acinetobacter baumannii ~3-fold by 48 hours. Conclusions: The combination of therapeutics used has a significant role in biofilm prevention for selected strains via direct contact or diffusion in aqueous solutions.

Keywords