Transient Gene Expression for the Characteristic Signal Sequences and the Estimation of the Localization of Target Protein in Plant Cell
Mikhail Berestovoy,
Alexander Tyurin,
Ksenia Kabardaeva,
Yuriy Sidorchuk,
Artem Fomenkov,
Alexander Nosov,
Irina Goldenkova-Pavlova
Affiliations
Mikhail Berestovoy
Institute of Plant Physiology, Russian Academy of Sciences, Moscow, RussiaRussian State Agrarian University–Moscow Timiryazev Agricultural Academy, Moscow, Russia
Alexander Tyurin
Institute of Plant Physiology, Russian Academy of Sciences, Moscow, RussiaRussian State Agrarian University–Moscow Timiryazev Agricultural Academy, Moscow, Russia
Ksenia Kabardaeva
Institute of Plant Physiology, Russian Academy of Sciences, Moscow, RussiaRussian State Agrarian University–Moscow Timiryazev Agricultural Academy, Moscow, Russia
Yuriy Sidorchuk
Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, Novosibirsk, Russia
Artem Fomenkov
Institute of Plant Physiology, Russian Academy of Sciences, Moscow, Russia
Alexander Nosov
Institute of Plant Physiology, Russian Academy of Sciences, Moscow, Russia
Irina Goldenkova-Pavlova
Institute of Plant Physiology, Russian Academy of Sciences, Moscow, Russia
We have proposed and tested a method for characterization of the signal sequences and determinations of target protein localization in a plant cell. This method, called the AgI-PrI, implies extraction of protoplasts from plant tissues after agroinfiltration. The suggested approach combines the advantages of two widely used methods for transient gene expression in plants–agroinfiltration and transfection of isolated protoplasts. The AgI-PrI technic can be applied to other plant species.
