Frontiers in Immunology (Aug 2024)
Expression of IL-1β in transgenic Eimeria necatrix enhances the immunogenicity of parasites and promotes mucosal immunity against coccidiosis
- Chunhui Duan,
- Chunhui Duan,
- Chunhui Duan,
- Tumalisi Abudureheman,
- Tumalisi Abudureheman,
- Tumalisi Abudureheman,
- Si Wang,
- Si Wang,
- Si Wang,
- Jingxia Suo,
- Jingxia Suo,
- Jingxia Suo,
- Ying Yu,
- Ying Yu,
- Ying Yu,
- Fangyun Shi,
- Fangyun Shi,
- Fangyun Shi,
- Xianyong Liu,
- Xianyong Liu,
- Xianyong Liu,
- Dina B. Salama,
- Dina B. Salama,
- Dina B. Salama,
- Dina B. Salama,
- Ratnesh Kumar Srivastav,
- Nishith Gupta,
- Nishith Gupta,
- Xun Suo,
- Xun Suo,
- Xun Suo
Affiliations
- Chunhui Duan
- National Key Laboratory of Veterinary Public Health Security, College of Veterinary Medicine, China Agricultural University, Beijing, China
- Chunhui Duan
- Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, China Agricultural University, Beijing, China
- Chunhui Duan
- National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing, China
- Tumalisi Abudureheman
- National Key Laboratory of Veterinary Public Health Security, College of Veterinary Medicine, China Agricultural University, Beijing, China
- Tumalisi Abudureheman
- Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, China Agricultural University, Beijing, China
- Tumalisi Abudureheman
- National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing, China
- Si Wang
- National Key Laboratory of Veterinary Public Health Security, College of Veterinary Medicine, China Agricultural University, Beijing, China
- Si Wang
- Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, China Agricultural University, Beijing, China
- Si Wang
- National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing, China
- Jingxia Suo
- National Key Laboratory of Veterinary Public Health Security, College of Veterinary Medicine, China Agricultural University, Beijing, China
- Jingxia Suo
- Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, China Agricultural University, Beijing, China
- Jingxia Suo
- National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing, China
- Ying Yu
- National Key Laboratory of Veterinary Public Health Security, College of Veterinary Medicine, China Agricultural University, Beijing, China
- Ying Yu
- Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, China Agricultural University, Beijing, China
- Ying Yu
- National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing, China
- Fangyun Shi
- National Key Laboratory of Veterinary Public Health Security, College of Veterinary Medicine, China Agricultural University, Beijing, China
- Fangyun Shi
- Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, China Agricultural University, Beijing, China
- Fangyun Shi
- National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing, China
- Xianyong Liu
- National Key Laboratory of Veterinary Public Health Security, College of Veterinary Medicine, China Agricultural University, Beijing, China
- Xianyong Liu
- Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, China Agricultural University, Beijing, China
- Xianyong Liu
- National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing, China
- Dina B. Salama
- Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, China Agricultural University, Beijing, China
- Dina B. Salama
- National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing, China
- Dina B. Salama
- Intracellular Parasite Education And Research Labs (iPEARL), Department of Biological Sciences, Birla Institute of Technology and Science, Pilani (BITS-P), Hyderabad, India
- Dina B. Salama
- Parasitology and Animal Disease Department, Veterinary Research Institute, National Research Centre, Giza, Egypt
- Ratnesh Kumar Srivastav
- Intracellular Parasite Education And Research Labs (iPEARL), Department of Biological Sciences, Birla Institute of Technology and Science, Pilani (BITS-P), Hyderabad, India
- Nishith Gupta
- Intracellular Parasite Education And Research Labs (iPEARL), Department of Biological Sciences, Birla Institute of Technology and Science, Pilani (BITS-P), Hyderabad, India
- Nishith Gupta
- Department of Molecular Parasitology, Institute of Biology, Faculty of Life Sciences, Humboldt University, Berlin, Germany
- Xun Suo
- National Key Laboratory of Veterinary Public Health Security, College of Veterinary Medicine, China Agricultural University, Beijing, China
- Xun Suo
- Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, China Agricultural University, Beijing, China
- Xun Suo
- National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing, China
- DOI
- https://doi.org/10.3389/fimmu.2024.1435702
- Journal volume & issue
-
Vol. 15
Abstract
Anticoccidial vaccines comprising living oocysts of Eimeria tenella, Eimeria necatrix, Eimeria maxima, and Eimeria acervulina are used to control coccidiosis. This study explored the potential of IL-1β to act as a molecular adjuvant for enhancing the immunogenicity of Eimeria necatrix and mucosal immunity. We engineered E. necatrix to express a functional chIL-1β (EnIL-1β) and immunized chickens with oocysts of the wild type (EnWT) and tranegenic (EnIL-1β) strains, respectively. The chickens were then challenged with EnWT oocysts to examine the immunogenicity-enhancing potential of chIL-1β. As expected, the oocyst output of EnIL-1β-immunized chickens was significantly reduced compared to those immunized using EnWT. No difference in body weight gain and lesion scores of EnIL-1β and EnWT groups was observed. The parasite load in the small intestine and caeca showed that the invasion and replication of EnIL-1β was not affected. However, the markers of immunogenicity and mucosal barrier, Claudin-1 and avian β-defensin-1, were elevated in EnIL-1β-infected chickens. Ectopic expression of chIL-1β in E. necatrix thus appears to improve its immunogenicity and mucosal immunity, without increasing pathogenicity. Our findings support chIL-1β as a candidate for development of effective live-oocyst-based anticoccidial vaccines.
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