The Wound-Healing Effect of a Novel Fibroblasts-Impregnated Hydroxyethylcellulose Gel in a Rat Full-Thickness Burn Model: A Preclinical Study
Yury A. Novosad,
Anton S. Shabunin,
Natella I. Enukashvily,
Olga V. Supilnikova,
Anastasia I. Konkina,
Natalia Yu. Semenova,
Gleb S. Yatsemirsky,
Evgenii V. Zinoviev,
Kristina N. Rodionova,
Kirill L. Kryshen,
Antonina Yu. Borodina,
Alexander Yu. Makarov,
Andrey M. Fedyuk,
Alexander D. Nilov,
Elena V. Chikulaeva,
Lidiya S. Konkova,
Irina S. Chustrak,
Veronika V. Traxova,
Platon A. Safonov,
Sergey V. Vissarionov,
Egor M. Prikhodko,
Yury V. Yurkevich
Affiliations
Yury A. Novosad
Professor G.I. Gaivoronsky Laboratory of Experimental Traumatology and Orthopedics with Vivarium, H. Turner National Medical Research Center for Children’s Orthopedics and Trauma Surgery, 196603 St. Petersburg, Russia
Anton S. Shabunin
Professor G.I. Gaivoronsky Laboratory of Experimental Traumatology and Orthopedics with Vivarium, H. Turner National Medical Research Center for Children’s Orthopedics and Trauma Surgery, 196603 St. Petersburg, Russia
Natella I. Enukashvily
Cell Technology Center Pokrovsky, 199066 St. Petersburg, Russia
Olga V. Supilnikova
Cell Technology Center Pokrovsky, 199066 St. Petersburg, Russia
Anastasia I. Konkina
Cell Technology Center Pokrovsky, 199066 St. Petersburg, Russia
Natalia Yu. Semenova
Research Department of Pathomorphology, Center for Preclinical and Translational Research, Federal State Budgetary Institution «Almazov National Medical Research Centre», Ministry of Health of Russia, 199034 St. Petersburg, Russia
Gleb S. Yatsemirsky
Cell Technology Center Pokrovsky, 199066 St. Petersburg, Russia
Evgenii V. Zinoviev
Saint-Petersburg I. I. Dzhanelidze Research Institute of Emergency Medicine, 192242 St. Petersburg, Russia
Kristina N. Rodionova
Professor G.I. Gaivoronsky Laboratory of Experimental Traumatology and Orthopedics with Vivarium, H. Turner National Medical Research Center for Children’s Orthopedics and Trauma Surgery, 196603 St. Petersburg, Russia
Kirill L. Kryshen
“Home of Pharmacy” Center, Leningrad Region, 188663 Kuzmolovsky, Russia
Antonina Yu. Borodina
“Home of Pharmacy” Center, Leningrad Region, 188663 Kuzmolovsky, Russia
Alexander Yu. Makarov
Professor G.I. Gaivoronsky Laboratory of Experimental Traumatology and Orthopedics with Vivarium, H. Turner National Medical Research Center for Children’s Orthopedics and Trauma Surgery, 196603 St. Petersburg, Russia
Andrey M. Fedyuk
Professor G.I. Gaivoronsky Laboratory of Experimental Traumatology and Orthopedics with Vivarium, H. Turner National Medical Research Center for Children’s Orthopedics and Trauma Surgery, 196603 St. Petersburg, Russia
Alexander D. Nilov
Professor G.I. Gaivoronsky Laboratory of Experimental Traumatology and Orthopedics with Vivarium, H. Turner National Medical Research Center for Children’s Orthopedics and Trauma Surgery, 196603 St. Petersburg, Russia
Elena V. Chikulaeva
Professor G.I. Gaivoronsky Laboratory of Experimental Traumatology and Orthopedics with Vivarium, H. Turner National Medical Research Center for Children’s Orthopedics and Trauma Surgery, 196603 St. Petersburg, Russia
Lidiya S. Konkova
Professor G.I. Gaivoronsky Laboratory of Experimental Traumatology and Orthopedics with Vivarium, H. Turner National Medical Research Center for Children’s Orthopedics and Trauma Surgery, 196603 St. Petersburg, Russia
Irina S. Chustrak
Professor G.I. Gaivoronsky Laboratory of Experimental Traumatology and Orthopedics with Vivarium, H. Turner National Medical Research Center for Children’s Orthopedics and Trauma Surgery, 196603 St. Petersburg, Russia
Veronika V. Traxova
Professor G.I. Gaivoronsky Laboratory of Experimental Traumatology and Orthopedics with Vivarium, H. Turner National Medical Research Center for Children’s Orthopedics and Trauma Surgery, 196603 St. Petersburg, Russia
Platon A. Safonov
Professor G.I. Gaivoronsky Laboratory of Experimental Traumatology and Orthopedics with Vivarium, H. Turner National Medical Research Center for Children’s Orthopedics and Trauma Surgery, 196603 St. Petersburg, Russia
Sergey V. Vissarionov
H. Turner National Medical Research Center for Children’s Orthopedics and Trauma Surgery, 196603 St. Petersburg, Russia
Egor M. Prikhodko
Cell Technology Center Pokrovsky, 199066 St. Petersburg, Russia
Yury V. Yurkevich
Cell Technology Center Pokrovsky, 199066 St. Petersburg, Russia
Background/Objectives: The objective of this study was to assess the efficacy of a cell-containing wound dressing based on fibroblasts in hydroxyethylcellulose (HEC) gel for the local treatment of deep partial-thickness and/or full-thickness skin burns in an animal model. Methods: The rats (male Wistar, n = 100) were subjected to a full-thickness thermal burn (16 cm2). Radical necrectomy was performed one day after the burn. Three days later, the rats were randomly assigned to one of four groups: group 1 (no treatment), group 2 (chloramphenicol and methyluracil ointment, a routine clinical treatment), group 3 (a gel without cells, mock treatment), and group 4 (a dermal fibroblast-impregnated HEC gel). The treatment lasted for five days. The wound-healing process was evaluated by planimetric, cytologic, histologic, and immunohistochemical methods. Results: The differences in the rate of wound healing and the characteristics of wound cytology were identified. In the group 4, a regenerative type of cytogram was revealed, characterized by a significantly increased number of fibroblastic cells in comparison to samples from non-treated and mock-treated animals. Biopsy samples of burn wounds from animals in the group 4l demonstrated the presence of mature granulation tissue and a large number of microvessels. The repair process was stimulated, as evidenced by the increased thickness of newly formed granulation tissue and epidermis in the wound zone, elevated cellularity, and enhanced re-epithelialization activity. The number of Ki-67-positive proliferating cells was significantly higher in group 4 than in the control groups). A small number of non-proliferating donor fibroblasts was observed in the wound area 3 days after the end of treatment. Conclusions: The cell product is an effective agent for promoting wound healing during the regenerative phase. The experiments demonstrated that a gel populated by dermal fibroblasts can stimulate reparative regeneration processes in deep partial- and full-thickness burn wounds.
