Crystal structure of the cobalt human insulin derivative

Abstract

The structure of the human cobalt insulin derivative at 1.73 Å resolution is described. Single were prepared by the hanging drop vapour diffusion crystallisation method using Zn-free insulin and cobalt(II) acetate. The crystal structure was determined by the single crystal X-ray diffraction method. The investigated insulin derivative exhibits the T6 form of insulin and crystallizes in the trigonal system in space group R3, with the unit cell parameters a = b = 81.43 Å and c = 33.75 Å. The two cobalt atoms per insulin hexamer and are octahedrally coordinated by three symmetry-related Nε2 atoms of three HisB10/HisD10 and three oxygen atoms from three water molecules.

Keywords

• insulin derivative, cobalt, x-ray structure