Use of Human Intestinal Enteroids to Detect 
Human Norovirus Infectivity

Abstract

Tools to detect human norovirus infectivity have been lacking. Using human intestinal enteroid cultures inoculated with GII.Pe-GII.4 Sydney–infected fecal samples, we determined that a real-time reverse transcription PCR cycle threshold cutoff of 30 may indicate infectious norovirus. This finding could be used to help guide infection control.

Keywords

• Cycle threshold
• Ct cutoff
• enteroids
• infectivity
• human norovirus
• rRT-PCR