The influence of different doses of mineral nitrogen on total protein content of roots and nodules of soybean, inoculated by strains of Bradyrhizobium aponicum with contrasting symbiotic features

Abstract

To investigate the dynamics of changes in total protein content of soybeans roots and nodules under inoculation by Bradyrhizobium japonicum strains with contrasting symbiotic characteristics and under different providing of mineral nitrogen. Seeds were inoculated by active and inactive strains of rhizobia. Soybean plants were grown under conditions of vegetation experiment. To obtain protein extracts roots and nodules were collected at the stages of budding, beginning flowering and full flowering of plants. The tootal protein content in the samples was determined by the method of Bradford. It was shown the changes of total protein content in the soybean roots and nodules, depending on inoculation of strains of B.japonicum, the phase of plant growth and the amount of mineral nitrogen.

Keywords

• nitrogen fixation, rhizobia, soybean, symbiotic systems, proteins