Biological and Physico-Chemical Properties of Composite Layers Based on Magnesium-Doped Hydroxyapatite in Chitosan Matrix
Simona Liliana Iconaru,
Carmen Steluta Ciobanu,
Gabriel Predoi,
Krzysztof Rokosz,
Mariana Carmen Chifiriuc,
Coralia Bleotu,
George Stanciu,
Radu Hristu,
Steinar Raaen,
Stefania Mariana Raita,
Liliana Ghegoiu,
Monica Luminita Badea,
Daniela Predoi
Affiliations
Simona Liliana Iconaru
National Institute of Materials Physics, 405A Atomistilor Street, 077125 Magurele, Romania
Carmen Steluta Ciobanu
National Institute of Materials Physics, 405A Atomistilor Street, 077125 Magurele, Romania
Gabriel Predoi
Faculty of Veterinary Medicine, University of Agronomic Sciences and Veterinary Medicine of Bucharest, 105 Splaiul Independentei, 050097 Bucharest, Romania
Krzysztof Rokosz
Faculty of Electronics and Computer Science, Koszalin University of Technology, Sniadeckich 2, PL 75-453 Koszalin, Poland
Mariana Carmen Chifiriuc
Life, Environmental and Earth Sciences Division, Research Institute of the University of Bucharest (ICUB), University of Bucharest, 060023 Bucharest, Romania
Coralia Bleotu
Life, Environmental and Earth Sciences Division, Research Institute of the University of Bucharest (ICUB), University of Bucharest, 060023 Bucharest, Romania
George Stanciu
Center for Microscopy-Microanalysis and Information Processing, University Politehnica of Bucharest, 313 Splaiul Independentei, 060042 Bucharest, Romania
Radu Hristu
Center for Microscopy-Microanalysis and Information Processing, University Politehnica of Bucharest, 313 Splaiul Independentei, 060042 Bucharest, Romania
Steinar Raaen
Department of Physics, Norwegian University of Science and Technology (NTNU), Realfagbygget E3-124 Høgskoleringen 5, NO 7491 Trondheim, Norway
Stefania Mariana Raita
Faculty of Veterinary Medicine, University of Agronomic Sciences and Veterinary Medicine of Bucharest, 105 Splaiul Independentei, 050097 Bucharest, Romania
Liliana Ghegoiu
National Institute of Materials Physics, 405A Atomistilor Street, 077125 Magurele, Romania
Monica Luminita Badea
National Institute of Materials Physics, 405A Atomistilor Street, 077125 Magurele, Romania
Daniela Predoi
National Institute of Materials Physics, 405A Atomistilor Street, 077125 Magurele, Romania
In the present study, we report the development and characterization of composite layers (by spin coating) based on magnesium-doped hydroxyapatite in a chitosan matrix, (Ca10−xMgx(PO4)6(OH)2; xMg = 0, 0.08 and 0.3; HApCh, 8MgHApCh and 30MgHApCh). The MgHApCh composite layers were investigated using scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), and X-ray photoelectron spectroscopy (XPS) techniques. The in vitro biological evaluation included the assessment of their cytotoxicity on MG63 osteoblast-like cells and antifungal activity against Candida albicans ATCC 10231 fungal cell lines. The results of the physico-chemical characterization highlighted the obtaining of uniform and homogeneous composite layers. In addition, the biological assays demonstrated that the increase in the magnesium concentration in the samples enhanced the antifungal effect but also decreased their cytocompatibility. However, for certain optimal magnesium ion concentrations, the composite layers presented both excellent biocompatibility and antifungal properties, suggesting their promising potential for biomedical applications in both implantology and dentistry.
