Minimal Inhibitory Concentration Assay of Tridax procumbens Stem Extract-Based Chitosan Gel against Pseudomonas aeruginosa

Abstract

Introduction: Tridax procumbens has long been used as an insect deterrent, anticoagulant, and for the healing of wounds. In some areas of India, traditional healers also employ T. procumbens to treat injuries, blisters, and boils. A widespread rod-shaped, gram-negative, strictly aerobic bacteria called Pseudomonas aeruginosa can infect both plants and animals, including humans. It can be found in a variety of infections, particularly those that have a tendency to become chronic such as gingivitis and periodontitis. This study aims to assess the minimum inhibitory concentration (MIC) assay of T. procumbens stem extract-based chitosan gel against P. aeruginosa. Materials and Methods: The extract was produced from powdered T. procumbens stem part. To create the wound-healing gel, medium molecular weight chitosan was added to the T. procumbens stem extract to form a homogenous gel. Time-kill assay was conducted to determine the time-kill kinetics of the T. procumbens stem extract-based chitosan gel against P. aeruginosa. Results: The absorbance values decreased progressively over time at all concentrations (25 μL, 50 μL, and 100 μL) showing its inhibitory effect. With the increase in concentration, the inhibitory effect of T. procumbens stem extract-based chitosan gel against P. aeruginosa also increased. Conclusion: T. procumbens stem extract-based chitosan gel has revealed potent antimicrobial activity against P. aeruginosa and therefore the gel can be applied for efficient wound healing.

Keywords

• antimicrobial activity
• chitosan gel
• disease
• innovation
• minimal inhibitory concentration
• t. procumbens