Nature Communications (Sep 2022)

Structure-function analysis of enterovirus protease 2A in complex with its essential host factor SETD3

  • Christine E. Peters,
  • Ursula Schulze-Gahmen,
  • Manon Eckhardt,
  • Gwendolyn M. Jang,
  • Jiewei Xu,
  • Ernst H. Pulido,
  • Conner Bardine,
  • Charles S. Craik,
  • Melanie Ott,
  • Or Gozani,
  • Kliment A. Verba,
  • Ruth Hüttenhain,
  • Jan E. Carette,
  • Nevan J. Krogan

DOI
https://doi.org/10.1038/s41467-022-32758-3
Journal volume & issue
Vol. 13, no. 1
pp. 1 – 15

Abstract

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Actin histidine methyltransferase SETD3 is a host factor critical for the replication of enteroviruses. Here, the authors report the 3.5 Å cryoEM structure of SETD3 interacting with enterovirus CV-B3 2A protease, defining the actin-binding SET domain as essential for virus replication.