Antibiotic sensitivity of Vibrio cholerae nonO1/nonO139 strains isolated from hydroecosystems in 2016-2017 in Rostov-on-Don

Abstract

Aim. To assess the prevalence of antimicrobial resistance of Vibrio cholerae nonO1/nonO139 strains in water bodies in Rostov-on-Don. Materials and methods. Sampling was carried out at fixed points of open reservoirs in Rostov-on-Don from May to September in 2016 and 2017. Sensitivity/resistance of V. cholerae nonO1/nonO139 to antibacterial drugs (ABP) recommended for emergency prevention and treatment of cholera was determined by serial dilution on Müller-Hinton agar. Results. 196 strains of V. cholerae nonO1/ nonO139 were isolated in 2016, 75 — in 2017. When serotyping it was found that all strains were assigned to 17 serogroups, representatives of O16 and O76 serogroups prevailed. Analysis of the distribution of strains on resistance to ABP showed that sensitive strains of V. cholerae nonO1/nonO139 were not detected. All microorganisms were resistant to furazolidone. The proportions of isolates resistant to co-trimoxazole and ampicillin were 35.2% and 40% in 2016, and 33.6% and 28.3% in 2017 respectively. All microorganisms were distributed over ten phenotypes and were characterized by the presence of 1 to 4 determinants of resistance. Conclusion. The resistance of V. cholerae nonO1/nonO139 strains isolated in Rostov-on-Don water bodies to furazolidone increased from 47% in 2011 — 2014 to 100% in 2016 — 2017. Resistance to co-trimaxazole increased from 7.8% to 35.2 — 40%. Strains resistant to gentamicin (1.5%) earlier absent were detected in 2016.

Keywords

• monitoring
• vibrio cholerae nono1/nono139 serogroups
• antibiotic resistance