Antioxidant potential and in vitro inhibition of starch digestion of flavonoids from Crataegus pinnatifida

Abstract

Hawthorn flavonoids were extracted by enzymolysis associated ultrasonic procedure. Thirteen flavonoids were identified by HPLC/ESI-QTOF/MS, and the major components were procyanidin C1, rutin-rhamnoside, vitexin-rhamnoside, and catechin. Hawthorn flavonoids exhibited strong free radical scavenging activities against DPPH, ABTS, and hydroxyl radicals. Total and intercellular antioxidant experiments revealed that the free hydro-PSC value was 295.32 ± 12.20 μmol of VCE/g DW, and the free cellular antioxidant activity (CAA) values were 168.60 ± 4.87 μmol of QE/g DW in the no PBS wash protocol and 49.53 ± 1.75 μmol of QE/g DW in the PBS wash protocol. In addition, hawthorn flavonoids exhibited higher α-amylase and α-glucosidase inhibitory activities. The wheat starch digestibility was also reduced by hawthorn flavonoids as well. The results indicated that enzymolysis associated ultrasonic extraction was advisable for extracting flavonoids from hawthorn, and hawthorn flavonoids might be recommended as a potential food supplement with hypoglycemic activities.

Keywords

• Flavonoids
• Antioxidant activity
• α-Amylase
• α-Glucosidase
• Starch digestion