Annals of Clinical Microbiology and Antimicrobials (Dec 2021)

Epidemiological profile of multidrug-resistant and extensively drug-resistant Mycobacterium Tubrculosis among Congolese patients

  • Darrel Ornelle Elion Assiana,
  • Jabar Babatunde Pacôme Achimi Abdul,
  • Laure Stella Ghoma Linguissi,
  • Micheska Epola,
  • Jeannhey Christevy Vouvoungui,
  • Albert Mabiala,
  • Christopher Mebiame Biyogho,
  • Jean Ronald Edoa,
  • Bayodé Roméo Adegbite,
  • Ayola Akim Adegnika,
  • Linzy Elton,
  • Julio Ortiz Canseco,
  • Timothy D. McHugh,
  • Gabriel Ahombo,
  • Francine Ntoumi

DOI
https://doi.org/10.1186/s12941-021-00488-x
Journal volume & issue
Vol. 20, no. 1
pp. 1 – 8

Abstract

Read online

Abstract Background There is paucity of data on the prevalence and distribution of multidrug- Resistant-Tuberculosis (MDR-TB) in the Republic of Congo. Among the challenges resides the implementation of a robust TB resistance diagnostic program using molecular tools. In resource limited settings there is a need to gather data to enable prioritization of actions. The objective of this study was is to implement molecular tools as a best of diagnosing MDR and XDR-TB among presumptive tuberculosis patients referred to reference hospital of Makelekele in Brazzaville, Republic of the Congo. Methods We have conducted a cross-sectional study, including a total of 92 presumptive pulmonary tuberculosis patients and who had never received treatment recruited at the reference hospital of Makelekele from October 2018 to October 2019. The socio-demographic and clinical data were collected as well as sputum samples. Rifampicin resistance was investigated using Xpert (Cepheid) and second-line TB drugs Susceptibility testing were performed by the Brucker HAIN Line Probe Assay (GenoType MTBDRsl VER 2.0 assay) method. Results From the 92 recruited patients, 57 (62%) were found positive for the Mycobacterium tuberculosis complex. The prevalence of rifampicin-resistant tuberculosis (RR-TB) was 9.8% (9/92) and importantly 2.2% were pre-XDR/XDR. Conclusion This study showed a high rate of rifampicin resistance and the presence of extensively drug-resistant tuberculosis in the study area in new patients. This study highlights the need for further studies of TB drug resistance in the country.

Keywords