Transcriptome Profiling Reveals Molecular Changes during Flower Development between Male Sterile and Fertile Chinese Cabbage (<i>Brassica</i> <i>rapa</i> ssp. <i>pekinensis</i>) Lines
Jingfeng Hu,
Mei Lan,
Xuezhong Xu,
Hongli Yang,
Liqin Zhang,
Fengxian Lv,
Huiju Yang,
Ding Yang,
Chongjuan Li,
Jiangming He
Affiliations
Jingfeng Hu
Institute of Horticultural Crops, Yunnan Academy of Agricultural Sciences, Yunnan Branch of the National Vegetable Improvement Center, Kunming 650205, China
Mei Lan
Institute of Horticultural Crops, Yunnan Academy of Agricultural Sciences, Yunnan Branch of the National Vegetable Improvement Center, Kunming 650205, China
Xuezhong Xu
Institute of Horticultural Crops, Yunnan Academy of Agricultural Sciences, Yunnan Branch of the National Vegetable Improvement Center, Kunming 650205, China
Hongli Yang
Institute of Horticultural Crops, Yunnan Academy of Agricultural Sciences, Yunnan Branch of the National Vegetable Improvement Center, Kunming 650205, China
Liqin Zhang
Institute of Horticultural Crops, Yunnan Academy of Agricultural Sciences, Yunnan Branch of the National Vegetable Improvement Center, Kunming 650205, China
Fengxian Lv
State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan & School of Life Sciences, Yunnan University, Kunming 650091, China
Huiju Yang
Lijiang Teachers College, Lijiang 674100, China
Ding Yang
State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan & School of Life Sciences, Yunnan University, Kunming 650091, China
Chongjuan Li
State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan & School of Life Sciences, Yunnan University, Kunming 650091, China
Jiangming He
Institute of Horticultural Crops, Yunnan Academy of Agricultural Sciences, Yunnan Branch of the National Vegetable Improvement Center, Kunming 650205, China
Male sterility exists widely in flowering plants and is used as a fascinating tool by breeders for creating hybrid varieties. Herein, stamen samples from male sterile CCR20000 and male fertile CCR20001 lines during two developmental stages were employed to elucidate the molecular changes during flower development in fertile and sterile Chinese cabbage lines. RNA-seq revealed weak transcriptional activity in the sterile line, which may have led to the abnormal stamen development. The differentially expressed genes were enriched in plant hormone, carbon metabolism, and biosynthesis of amino acid pathways. Important genes with opposite patterns of regulation between the two lines have been associated with the male sterility trait. Members of the transcription factor families such as AP2, MYB, bHLH, and WRKY were highly active in the regulation of structural genes involved in pollen fertility. This study generated important genomic information to support the exploitation of the male sterility trait in Chinese cabbage breeding programs.
