Evaluation of essential oils nanoemulsions formulations on Botrytis cinerea growth, pathology and grey mould incidence on cucumber fruits

Abstract

Abstract Background Grey mould caused by Botrytis cinerea of cucumber immature fruits is the most epidemic fungal diseases causing significantly losses of fruits during development on cucumber plant in winter season. Material and methods Crude and nanoemulsions of clove, black seed, lemon and orange essential oils at (5000 ppm) and also different formulations of clove, black seed and lemon oils nanoemulsions as single or in combination were tested against mycelial linear growth of B. cinerea (MF996363) as well sclerotial formation and pathological activity on the development of germinated seeds of cucumber. Results Essential oils nanoemulsion formulation of clove + black seed (2:1) at 5000 ppm was the best formulation significantly reduced mycelia linear growth of B. cinerea by 61.0% followed by 28% formulation (1:1) and (1:2). In addition, nanoemulsions formulations of clove, black seed essential oils (2:1) were highly reduced the count of sclerotial formation of B. cinerea and completely (100%) suppress seed rot and seedling mortality of cucumber followed by nanoemulsion of clove only. Conclusions Foliar application of nanoemulsion formulation of clove + black seeds is promising than fungicides (Topsin M-70) in controlling grey mould on cucumber fruits caused by B. cinerea in plastic greenhouse with no phytotoxicity on cucumber plants.

Keywords

• Cucumber
• Botrytis cinerea
• Grey mould
• Nanoemulsion
• Oils