A Simple Microfluidic Platform for Long-Term Analysis and Continuous Dual-Imaging Detection of T-Cell Secreted IFN-γ and IL-2 on Antibody-Based Biochip
Dieudonné R. Baganizi,
Loïc Leroy,
Loïc Laplatine,
Stacie J. Fairley,
Samuel Heidmann,
Samia Menad,
Thierry Livache,
Patrice N. Marche,
Yoann Roupioz
Affiliations
Dieudonné R. Baganizi
University of Grenoble Alpes, INAC-SPRAM, Grenoble F-38000, France
Loïc Leroy
University of Grenoble Alpes, INAC-SPRAM, Grenoble F-38000, France
Loïc Laplatine
University of Grenoble Alpes, INAC-SPRAM, Grenoble F-38000, France
Stacie J. Fairley
University of Grenoble Alpes, INAC-SPRAM, Grenoble F-38000, France
Samuel Heidmann
University of Grenoble Alpes, INAC-SPRAM, Grenoble F-38000, France
Samia Menad
University of Grenoble Alpes, INAC-SPRAM, Grenoble F-38000, France
Thierry Livache
University of Grenoble Alpes, INAC-SPRAM, Grenoble F-38000, France
Patrice N. Marche
University Grenoble Alpes, Institut Albert Bonniot, Grenoble F-38000, France
Yoann Roupioz
University of Grenoble Alpes, INAC-SPRAM, Grenoble F-38000, France
The identification and characterization, at the cellular level, of cytokine productions present a high interest for both fundamental research and clinical studies. However, the majority of techniques currently available (ELISA, ELISpot, flow cytometry, etc.) have several shortcomings including, notably, the assessment of several cytokines in relation to individual secreting cells and the monitoring of living cell responses for a long incubation time. In the present work, we describe a system composed of a microfluidic platform coupled with an antibody microarray chip for continuous SPR imaging and immunofluorescence analysis of cytokines (IL-2 and IFN-γ) secreted by T-Lymphocytes, specifically, and stably captured on the biochip under flow upon continued long-term on-chip culture (more than 24 h).
