The effectiveness of the use of the genetic marker F295 to assess the resistance of cucumber samples to the green mottled mosaic virus (CGMMV)

Abstract

Relevance. Cucumber green mottled mosaic virus (CGMMV) is a highly specialized virus (group Tobamovirus). It is a significant threat to cucurbits. On a high infectious background, cucumber yield losses, both in open and protected ground, can be close to 100%. The spread of the virus occurs in many ways. The most dangerous is the infected seed material. One of the effective methods of preventing CGMMV is the creation of genetically resistant hybrids to viral diseases. An experiment was conducted to determine the effectiveness of the use of the marker F295 for the selection of resistant samples.Results. When comparing the results of determining resistance against an artificial background and the results of PCR analysis for the same samples, we calculated the efficiency of the genetic marker F295, which was 65%. Since the level of effectiveness is average, for a more objective assessment, it is necessary to create another – the main marker, which will determine the direct presence of resistance to CGMMV. The use of the F295 marker is relevant in combination with other methods for deter[1]mining resistance, which will speed up and increase the efficiency of the breeding process.

Keywords

• cucumber
• parental lines
• cucumber green mottled mosaic virus cgmmv
• genetic markers
• pcr-analysis