The role of green peas protein hydrolysate in TGF/SMAD signaling to prevent renal fibrosis

Abstract

Objectives: The protein of green peas (Pisum sativum) hydrolyzed by bromelain (PHGPB) shows an effective renal fibrosis therapy by improving renal function parameters in the CKD rats model. The study aimed to determine anti-fibrotic properties effects of PHGPB and LERGDT on TGF-β1 level/SMAD 2,3,4 and 7 gene expressions in a fibrosis renal cell line model, glucose-induced MES13 SV40 cells. Methods: PHGPB was obtained using simple hydrolysis methods, LERGDT peptide was synthesized using Merryfield method, Chronic kidney model cells by glucose-induced MES13 SV40 cells. SMADs gene expression was counted using qRT-PCR, and TGF-β1 levels were measured by the ELISA method. Results: The result showed that treatment of 20 mM glucose induction in mesangial cells (kidney sclerosis model) increased the gene expression of SMAD2, SMAD3, and SMAD4, and decreased the expression of SMAD7 genes. Provision of PHGPB and LERGDT to kidney sclerosis cells models decreased the TGF-β1 level and gene expression of SMAD2 and SMAD4 genes at all concentrations, SMAD3 with 25 µg/mL PHGPB and LERGDT treatment. Gene expression of the SMAD7 increased after administration of PHGPB and LERGDT samples in all concentrations. Conclusion: The provision of PHGPB and LERGDT suppressed the expression of SMAD 2, 3, and 4 genes, yet increased the SMAD 7 gene. PHGPB and LERGDT have promising anti-fibrotic effects on TGF-β1 level/SMAD2, SMAD3, SMAD4, and SMAD7 gene expression in glucose-induced MES13 SV40 cells.

Keywords

• Pisum sativum
• TGF-β1
• SMAD2
• SMAD3
• SMAD 4
• SMAD 7