Polymorphism of prion protein gene (PRNP) in Nigerian sheep
Adeniyi C. Adeola,
Semiu F. Bello,
Abdussamad M. Abdussamad,
Akanbi I. Mark,
Oscar J. Sanke,
Anyebe B. Onoja,
Lotanna M. Nneji,
Nasiru Abdullahi,
Sunday C. Olaogun,
Lawal D. Rogo,
Godwin F. Mangbon,
Shamsudeen L. Pedro,
Manasseh P. Hiinan,
Muhammad M. Mukhtar,
Jebi Ibrahim,
Hayatu Saidu,
Philip M. Dawuda,
Rukayya K. Bala,
Hadiza L. Abdullahi,
Adebowale E. Salako,
Samia Kdidi,
Mohamed Habib Yahyaoui,
Ting-Ting Yin
Affiliations
Adeniyi C. Adeola
State Key Laboratory of Genetic Resources and Evolution & Yunnan Laboratory of Molecular Biology of Domestic Animals, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China
Semiu F. Bello
Department of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou, China
Abdussamad M. Abdussamad
Centre for Biotechnology Research, Bayero University, Kano, Nigeria
Akanbi I. Mark
Ministry of Agriculture and Rural Development, Secretariat, Ibadan, Nigeria
Oscar J. Sanke
Taraba State Ministry of Agriculture and Natural Resources, Jalingo, Nigeria
Anyebe B. Onoja
Department of Virology, College of Medicine, University of Ibadan, Ibadan, Nigeria
Lotanna M. Nneji
Department of Ecology and Evolutionary Biology, Princeton University, Princeton, NJ, USA
Nasiru Abdullahi
Department of Biochemistry, Faculty of Basic Medical Sciences, College of Health Sciences, Bayero University, Kano, Nigeria
Sunday C. Olaogun
Department of Veterinary Medicine, Faculty of Veterinary Medicine, University of Ibadan, Ibadan, Nigeria
Lawal D. Rogo
Department of Medical Laboratory Science, Faculty of Allied Health Sciences, College of Health Sciences, Bayero University, Kano, Nigeria
Godwin F. Mangbon
Division of Veterinary Office, Serti, Nigeria
Shamsudeen L. Pedro
Centre for Biotechnology Research, Bayero University, Kano, Nigeria
Manasseh P. Hiinan
Small Ruminant Section, Solomon Kesinton Agro-Allied Limited Iperu-Remo, Ogun State, Nigeria
Muhammad M. Mukhtar
Department of Biochemistry, Faculty of Basic Medical Sciences, College of Health Sciences, Bayero University, Kano, Nigeria
Jebi Ibrahim
Department of Veterinary Surgery and Theriogenology, College of Veterinary Medicine, University of Agriculture Makurdi, Makurdi, Nigeria
Hayatu Saidu
Department of Medical Laboratory Science, Faculty of Allied Health Sciences, College of Health Sciences, Bayero University, Kano, Nigeria
Philip M. Dawuda
Department of Animal Science, Faculty of Agriculture, National University of Lesotho, Lesotho, Southern Africa
Rukayya K. Bala
Centre for Biotechnology Research, Bayero University, Kano, Nigeria
Hadiza L. Abdullahi
Department of Veterinary Physiology and Biochemistry, Faculty of Veterinary Medicine, Bayero University, Kano, Nigeria
Adebowale E. Salako
Department of Animal Science, Faculty of Agriculture, University of Ibadan, Ibadan, Nigeria
Samia Kdidi
Livestock and Wildlife Laboratory, Institut des Régions Arides, Université de Gabes, Medenine, Tunisia
Mohamed Habib Yahyaoui
Livestock and Wildlife Laboratory, Institut des Régions Arides, Université de Gabes, Medenine, Tunisia
Ting-Ting Yin
State Key Laboratory of Genetic Resources and Evolution & Yunnan Laboratory of Molecular Biology of Domestic Animals, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China
ABSTRACTPolymorphism of the prion protein gene (PRNP) gene determines an animal’s susceptibility to scrapie. Three polymorphisms at codons 136, 154, and 171 have been linked to classical scrapie susceptibility, although many variants of PRNP have been reported. However, no study has investigated scrapie susceptibility in Nigerian sheep from the drier agro-climate zones. In this study, we aimed to identify PRNP polymorphism in nucleotide sequences of 126 Nigerian sheep by comparing them with public available studies on scrapie-affected sheep. Further, we deployed Polyphen-2, PROVEAN, and AMYCO analyses to determine the structure changes produced by the non-synonymous SNPs. Nineteen (19) SNPs were found in Nigerian sheep with 14 being non-synonymous. Interestingly, one novel SNP (T718C) was identified. There was a significant difference (P < 0.05) in the allele frequencies of PRNP codon 154 between sheep in Italy and Nigeria. Based on the prediction by Polyphen-2, R154H was probably damaging while H171Q was benign. Contrarily, all SNPs were neutral via PROVEAN analysis while two haplotypes (HYKK and HDKK) had similar amyloid propensity of PRNP with resistance haplotype in Nigerian sheep. Our study provides valuable information that could be possibly adopted in programs targeted at breeding for scrapie resistance in sheep from tropical regions.
