Sea cucumber (Holothuria glaberrima) intestinal microbiome dataset from Puerto Rico, generated by shotgun sequencing
Edwin Omar Rivera-Lopez,
Rene Nieves-Morales,
Gabriela Melendez-Martinez,
Jessica Alejandra Paez-Diaz,
Sofia Marie Rodriguez-Carrio,
Josue Rodriguez-Ramos,
Luis Morales-Valle,
Carlos Rios-Velazquez
Affiliations
Edwin Omar Rivera-Lopez
Microbial Biotechnology and Bioprospecting Laboratory, Biology Department, University of Puerto Rico, Mayagüez, P.R. 00681-9000, United States; Food Science and Technology Program, University of Puerto Rico at Mayagüez, P.R. 00681-9000, United States
Rene Nieves-Morales
Microbial Biotechnology and Bioprospecting Laboratory, Biology Department, University of Puerto Rico, Mayagüez, P.R. 00681-9000, United States
Gabriela Melendez-Martinez
Microbial Biotechnology and Bioprospecting Laboratory, Biology Department, University of Puerto Rico, Mayagüez, P.R. 00681-9000, United States
Jessica Alejandra Paez-Diaz
Microbial Biotechnology and Bioprospecting Laboratory, Biology Department, University of Puerto Rico, Mayagüez, P.R. 00681-9000, United States
Sofia Marie Rodriguez-Carrio
Microbial Biotechnology and Bioprospecting Laboratory, Biology Department, University of Puerto Rico, Mayagüez, P.R. 00681-9000, United States
Josue Rodriguez-Ramos
Pacific Northwest National Laboratory, Biological Sciences Division, WA, United States
Luis Morales-Valle
Microbial Biotechnology and Bioprospecting Laboratory, Biology Department, University of Puerto Rico, Mayagüez, P.R. 00681-9000, United States
Carlos Rios-Velazquez
Microbial Biotechnology and Bioprospecting Laboratory, Biology Department, University of Puerto Rico, Mayagüez, P.R. 00681-9000, United States; Corresponding author.
The sea cucumber (H. glaberrima) is a species found in the shallow waters near coral reefs and seagrass beds in Puerto Rico. To characterize the microbial taxonomic composition and functional profiles present in the sea cucumber, total DNA was obtained from their intestinal system, fosmid libraries constructed, and subsequent sequencing was performed. The diversity profile displayed that the most predominant domain was Bacteria (76.56 %), followed by Viruses (23.24 %) and Archaea (0.04 %). Within the 11 phyla identified, the most abundant was Proteobacteria (73.16 %), followed by Terrabacteria group (3.20 %) and Fibrobacterota, Chlorobiota, Bacteroidota (FCB) superphylum (1.02 %). The most abundant species were Porvidencia rettgeri (21.77 %), Pseudomonas stutzeri (14.78 %), and Alcaligenes faecalis (5.00 %). The functional profile revealed that the most abundant functions are related to transporters, MISC (miscellaneous information systems), organic nitrogen, energy, and carbon utilization. The data collected in this project on the diversity and functional profiles of the intestinal system of the H. glaberrima provided a detailed view of its microbial ecology. These findings may motivate comparative studies aimed at understanding the role of the microbiome in intestinal regeneration.
