Refinement of four major QTL for oil content in Brassica napus by integration of genome resequencing and transcriptomics

Shuxiang Yan; Huaixin Li; Hongbo Chao; Jianjie He; Yiran Ding; Weiguo Zhao; Kai Zhang; Yiyi Xiong; Kang Chen; Libin Zhang; Maoteng Li

Crop Journal (Jun 2022)

Refinement of four major QTL for oil content in Brassica napus by integration of genome resequencing and transcriptomics

Shuxiang Yan,
Huaixin Li,
Hongbo Chao,
Jianjie He,
Yiran Ding,
Weiguo Zhao,
Kai Zhang,
Yiyi Xiong,
Kang Chen,
Libin Zhang,
Maoteng Li

Affiliations

Shuxiang Yan: Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, Hubei, China; Key Laboratory of Molecular Biophysics of the Ministry of Education, Wuhan 430074, Hubei, China
Huaixin Li: Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, Hubei, China; Key Laboratory of Molecular Biophysics of the Ministry of Education, Wuhan 430074, Hubei, China
Hongbo Chao: Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, Hubei, China; School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, Henan, China
Jianjie He: Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, Hubei, China; Key Laboratory of Molecular Biophysics of the Ministry of Education, Wuhan 430074, Hubei, China
Yiran Ding: Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, Hubei, China; Key Laboratory of Molecular Biophysics of the Ministry of Education, Wuhan 430074, Hubei, China
Weiguo Zhao: Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, Hubei, China; Key Laboratory of Molecular Biophysics of the Ministry of Education, Wuhan 430074, Hubei, China
Kai Zhang: Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, Hubei, China; Key Laboratory of Molecular Biophysics of the Ministry of Education, Wuhan 430074, Hubei, China
Yiyi Xiong: Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, Hubei, China; Key Laboratory of Molecular Biophysics of the Ministry of Education, Wuhan 430074, Hubei, China
Kang Chen: Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, Hubei, China; Key Laboratory of Molecular Biophysics of the Ministry of Education, Wuhan 430074, Hubei, China
Libin Zhang: Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, Hubei, China; Key Laboratory of Molecular Biophysics of the Ministry of Education, Wuhan 430074, Hubei, China
Maoteng Li: Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, Hubei, China; Key Laboratory of Molecular Biophysics of the Ministry of Education, Wuhan 430074, Hubei, China; Corresponding author.

Journal volume & issue: Vol. 10, no. 3
pp. 627 – 637

Abstract

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Rapeseed (Brassica napus) supplies about half of the vegetable oil in China. Increasing oil production and searching for genes that control oil content in the crop are research goals. In our previous studies, four major QTL for oil content located on A08, A09, C03 and C06 in the KenC-8 × N53-2 (KN DH) mapping population were detected. The parental lines were resequenced to identify structural variations and candidate genes affecting oil content in these four major QTL regions. Insertion-deletion (InDel) markers were developed and used to narrow the regions. Differentially expressed genes located in the regions were investigated. GO and KEGG analysis showed that several genes were associated with lipid metabolism. Several transcription factors with higher expression in N53-2 than in KenC-8 were identified. These results shed light on the genetic control of oil content and may be helpful for the development of high-oil-content cultivars.

Published in Crop Journal

ISSN: 2095-5421 (Print); 2214-5141 (Online)
Publisher: KeAi Communications Co., Ltd.
Country of publisher: China
LCC subjects: Agriculture: Agriculture (General)
Website: https://www.keaipublishing.com/en/journals/the-crop-journal/

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