Rapid detection of fungal filaments in corneal scrapings by microwave heating-assisted grocott′s methenamine silver staining

Abstract

The Gomoris methanamine silver impregnation technique is a highly reliable and archiveable method of detecting fungal filaments, but the staining procedure is time consuming and laborious. A technique using microwave energy to reduce the duration of Gomori′s silver staining is described.

Keywords

• Fungus
• Corneal scrapings
• Microwave heating
• Gram′s stain
• Giemsa stain
• KOH staon/Calcofluor stain