Sperm Head Morphology Alterations Associated with Chromatin Instability and Lack of Protamine Abundance in Frozen-Thawed Sperm of Indonesian Local Bulls
Asmarani Kusumawati,
Faisal Amri Satrio,
Rhesti Indriastuti,
Zulfi Nur Amrina Rosyada,
Berlin Pandapotan Pardede,
Muhammad Agil,
Bambang Purwantara
Affiliations
Asmarani Kusumawati
Department of Reproduction and Obstetric, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia
Faisal Amri Satrio
Division of Reproduction and Obstetrics, School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor 16680, Indonesia
Rhesti Indriastuti
Tuah Sakato Technology and Resource Development Center, Department of Animal Husbandry and Animal Health of West Sumatra, Payakumbuh 26229, Indonesia
Zulfi Nur Amrina Rosyada
Research Center for Applied Zoology, National Research and Innovation Agency (BRIN), Bogor 16911, Indonesia
Berlin Pandapotan Pardede
Division of Reproduction and Obstetrics, School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor 16680, Indonesia
Muhammad Agil
Division of Reproduction and Obstetrics, School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor 16680, Indonesia
Bambang Purwantara
Division of Reproduction and Obstetrics, School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor 16680, Indonesia
This study aimed to analyze various alterations in the morphology of the sperm head and its association with nucleus instability and insufficient sperm protamine. Frozen-thawed semen from twenty local Indonesian bulls was used for all stages in this study. The results of sperm head defect assessments are used for bull grouping, high (HD) and low (LD). Sperm DNA damage was assessed using Acridine Orange and Halomax. The PRM1 protein abundance was carried out using an enzyme immunoassay, while PRM1 gene expression was carried out using the RT-qPCR. PRM deficiency was performed using CMA3. Several kinds of sperm head defects in the HD were significantly higher (p p p p p < 0.01) with sperm head defects, DNA damage, and PRM1 abundance. The lack of sperm protamine might affect the sperm nucleus’s stability and induce morphological alterations in the sperm head.
