Microarray analyses of the infant gut microbiota

Abstract

To detect the cause of germ free infant gut become colonization after birth and develops though the pregnancy for mother, 4 days, 10 days. 4 months, 1 year to 2 years is important, which has strong relationship with health and disease infant. This research was carried out at the experimental laboratory of molecular microbiology department at Norwegian University Of Life Science, College of of NMBU, Ås la Norge / Norway, during Jan 2015 to get more information about the development of microbiota in infant. The pattern of colonizers is different at the different age. The purify faecal sample DNA perform universal amplification of the 16Sr RNA to the determination of gut microbiota by the GA-map infant array and 454 pyrosequencing. The results showed implicating the Bifidobacteria and Firmicutes that present in great amount during the development of the infant gut microbiota with reach to peak at the age of several weeks. A temporal trend in the microbiota composition was also detected which indicates the usefulness of this method for determining the temporal dynamics of the infant gut development.

Keywords

• Microbiota
• Gut
• Fecal
• Infant