Characterization of an induced pluripotent stem cell line (NCHi013-A) from a 5-year-old male with pulmonary atresia with intact ventricular septum and a biventricular repair

Meghan Hanley; Matthew Alonzo; Shiqiao Ye; Yang Yu; Javier Contreras; Jade Hayden; Vidu Garg; Ming-Tao Zhao

Stem Cell Research (Oct 2024)

Characterization of an induced pluripotent stem cell line (NCHi013-A) from a 5-year-old male with pulmonary atresia with intact ventricular septum and a biventricular repair

Meghan Hanley,
Matthew Alonzo,
Shiqiao Ye,
Yang Yu,
Javier Contreras,
Jade Hayden,
Vidu Garg,
Ming-Tao Zhao

Affiliations

Meghan Hanley: Center for Cardiovascular Research, Abigail Wexner Research Institute, Nationwide Children’s Hospital, Columbus, OH 43215, USA; The Heart Center, Nationwide Children’s Hospital, Columbus, OH 43215, USA
Matthew Alonzo: Center for Cardiovascular Research, Abigail Wexner Research Institute, Nationwide Children’s Hospital, Columbus, OH 43215, USA; The Heart Center, Nationwide Children’s Hospital, Columbus, OH 43215, USA
Shiqiao Ye: Center for Cardiovascular Research, Abigail Wexner Research Institute, Nationwide Children’s Hospital, Columbus, OH 43215, USA; The Heart Center, Nationwide Children’s Hospital, Columbus, OH 43215, USA
Yang Yu: Center for Cardiovascular Research, Abigail Wexner Research Institute, Nationwide Children’s Hospital, Columbus, OH 43215, USA; The Heart Center, Nationwide Children’s Hospital, Columbus, OH 43215, USA
Javier Contreras: Center for Cardiovascular Research, Abigail Wexner Research Institute, Nationwide Children’s Hospital, Columbus, OH 43215, USA; The Heart Center, Nationwide Children’s Hospital, Columbus, OH 43215, USA
Jade Hayden: Center for Cardiovascular Research, Abigail Wexner Research Institute, Nationwide Children’s Hospital, Columbus, OH 43215, USA; The Heart Center, Nationwide Children’s Hospital, Columbus, OH 43215, USA
Vidu Garg: Center for Cardiovascular Research, Abigail Wexner Research Institute, Nationwide Children’s Hospital, Columbus, OH 43215, USA; The Heart Center, Nationwide Children’s Hospital, Columbus, OH 43215, USA; Department of Pediatrics, The Ohio State University College of Medicine, Columbus, OH 43210, USA; Dorothy M. Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical Center, Columbus, OH 43210, USA
Ming-Tao Zhao: Center for Cardiovascular Research, Abigail Wexner Research Institute, Nationwide Children’s Hospital, Columbus, OH 43215, USA; The Heart Center, Nationwide Children’s Hospital, Columbus, OH 43215, USA; Department of Pediatrics, The Ohio State University College of Medicine, Columbus, OH 43210, USA; Dorothy M. Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical Center, Columbus, OH 43210, USA; Corresponding author.

Journal volume & issue: Vol. 80
p. 103526

Abstract

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Pulmonary atresia with intact ventricular septum (PA/IVS) is a rare congenital heart defect that causes a significant decrease of blood outflow from the heart and is fatal if left untreated. iPSC line NCHi013-A was produced from peripheral blood mononuclear cells from a male child with PA/IVS using Sendai virus reprogramming. NCHi013-A displayed normal stem cell morphology, expressed markers for pluripotency, and presented ability to differentiate into cells of endoderm, ectoderm, and mesoderm lineages. The iPSC line also maintained normal karyotype, was validated for cell identity, and tested negative for transgenes and mycoplasma contamination.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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