Research progress of loop-mediated isothermal amplification in the detection of Salmonella for food safety applications
Linlin Zhuang,
Jiansen Gong,
Ping Zhang,
Di Zhang,
Ying Zhao,
Jianbo Yang,
Guofang Liu,
Yu Zhang,
Qiuping Shen
Affiliations
Linlin Zhuang
School of Animal Husbandry and Veterinary Medicine, Jiangsu Vocational College of Agriculture and Forestry
Jiansen Gong
Poultry Institute, Chinese Academy of Agricultural Sciences
Ping Zhang
Poultry Institute, Chinese Academy of Agricultural Sciences
Di Zhang
Poultry Institute, Chinese Academy of Agricultural Sciences
Ying Zhao
State Key Laboratory of Digital Medical Engineering, Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering and Basic Medicine Research and Innovation Center of Ministry of Education, Zhongda Hospital, Southeast University
Jianbo Yang
School of Animal Husbandry and Veterinary Medicine, Jiangsu Vocational College of Agriculture and Forestry
Guofang Liu
School of Animal Husbandry and Veterinary Medicine, Jiangsu Vocational College of Agriculture and Forestry
Yu Zhang
State Key Laboratory of Digital Medical Engineering, Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering and Basic Medicine Research and Innovation Center of Ministry of Education, Zhongda Hospital, Southeast University
Qiuping Shen
School of Animal Husbandry and Veterinary Medicine, Jiangsu Vocational College of Agriculture and Forestry
Abstract Salmonella, the prevailing zoonotic pathogen within the Enterobacteriaceae family, holds the foremost position in global bacterial poisoning incidents, thereby signifying its paramount importance in public health. Consequently, the imperative for expeditious and uncomplicated detection techniques for Salmonella in food is underscored. After more than two decades of development, loop-mediated isothermal amplification (LAMP) has emerged as a potent adjunct to the polymerase chain reaction, demonstrating significant advantages in the realm of isothermal amplification. Its growing prominence is evident in the increasing number of reports on its application in the rapid detection of Salmonella. This paper provides a systematic exposition of the technical principles and characteristics of LAMP, along with an overview of the research progress made in the rapid detection of Salmonella using LAMP and its derivatives. Additionally, the target genes reported in various levels, including Salmonella genus, species, serogroup, and serotype, are summarized, aiming to offer a valuable reference for the advancement of LAMP application in Salmonella detection. Finally, we look forward to the development direction of LAMP and expect more competitive methods to provide strong support for food safety applications.
