Development of a Novel Electrochemical Biosensor Based on Organized Mesoporous Carbon and Laccase for the Detection of Serotonin in Food Supplements

Abstract

Serotonin is a biogenic amine that has multiple roles in the human body and is mainly known as the happiness hormone. A new laccase (Lac)-based biosensor has been developed for the qualitative and quantitative determination of serotonin in three dietary supplements from three different manufacturers. The enzyme was immobilized on an organized mesoporous carbon-modified carbon screen-printed electrode (OMC-SPE) by the drop-and-dry method, the active surface being pretreated with glutaraldehyde. With the new biosensor, serotonin was selectively detected from different solutions. Square-wave voltammetry was the technique used for the quantitative determination of serotonin, obtaining a detection limit value of 316 nM and a quantification limit value of 948 nM in the linearity range of 0.1–1.2 µM. The pH for the determinations was 5.2; at this value, the biocatalytic activity of the laccase was optimal. At the same time, the electrochemical performance of the OMC-SPE/Lac biosensor was compared with that of the unmodified sensor, a performance that highlighted the superiority of the biosensor and the very important role of the enzyme in electrodetection. The results obtained from the quantitative determination of serotonin by square-wave voltammetry were compared with those from the FTIR method, revealing a very good correlation between the results obtained by the two quantitative determination methods.

Keywords

• serotonin
• laccase
• biosensor
• square-wave voltammetry
• food supplements