Bone morphogenetic protein-2 loaded poly(D,L-lactide-co-glycolide) microspheres enhance osteogenic potential of gelatin/hydroxyapatite/Î²-tricalcium phosphate cryogel composite for alveolar ridge augmentation

Hao-Chieh Chang; Connie Yang; Fang Feng; Feng-Huei Lin; Chi-Hwa Wang; Po-Chun Chang

Journal of the Formosan Medical Association (Dec 2017)

Bone morphogenetic protein-2 loaded poly(D,L-lactide-co-glycolide) microspheres enhance osteogenic potential of gelatin/hydroxyapatite/Î²-tricalcium phosphate cryogel composite for alveolar ridge augmentation

Hao-Chieh Chang,
Connie Yang,
Fang Feng,
Feng-Huei Lin,
Chi-Hwa Wang,
Po-Chun Chang

Affiliations

Hao-Chieh Chang: Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, Taipei, Taiwan; Department of Dentistry, National Taiwan University Hospital, Taipei, Taiwan
Connie Yang: Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, Taipei, Taiwan
Fang Feng: Department of Chemical and Biomolecular Engineering, College of Engineering, National University of Singapore, Singapore, Singapore; School of Chemistry, Biology and Materials Engineering, Suzhou University of Science and Technology, Suzhou, China
Feng-Huei Lin: Institute of Biomedical Engineering, College of Engineering, National Taiwan University, Taipei, Taiwan
Chi-Hwa Wang: Department of Chemical and Biomolecular Engineering, College of Engineering, National University of Singapore, Singapore, Singapore
Po-Chun Chang: Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, Taipei, Taiwan; Department of Dentistry, National Taiwan University Hospital, Taipei, Taiwan; Corresponding author. 1 Chang-Te St, Taipei 100, Taiwan.

Journal volume & issue: Vol. 116, no. 12
pp. 973 – 981

Abstract

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Background/Purpose: Sufficient bony support is essential to ensure the success of dental implant osseointegration. However, the reconstruction of vertical ridge deficiencies is still a major challenge for dental implants. This study introduced a novel treatment strategy by infusing poly(D,L-lactide-co-glycolide) (PLGA) microspheres encapsulating bone morphogenetic protein-2 (BMP-2) within a gelatin/hydroxyapatite/Î²-tricalcium phosphate (gelatin/HA/Î²-TCP) cryogel composite to facilitate supra-alveolar ridge augmentation. Methods: The gelatin scaffold was crosslinked using cryogel technique, and HA/Î²-TCP particles were mechanically entrapped to form the gelatin/HA/Î²-TCP composite. Co-axial electrohydrodynamic atomization technology was used to fabricate PLGA microspheres encapsulating BMP-2. The composites of gelatin/HA/Î²-TCP alone, with infusion of BMP-2 solution (BMPi) or microspheres (BMPm), were fixed on rat mandibles using a titanium mini-implant for 4 weeks, and the therapeutic efficiency was evaluated by micro-computed tomography, bone fluorochrome, and histology. Results: The gelatin/HA/Î²-TCP composite was homogenously porous, and BMP-2 was sustained release from the microspheres without initial burst release. Ridge augmentation was noted in all specimens treated with the gelatin/HA/Î²-TCP composite, and greater bone deposition ratio were noted in Groups BMPi and BMPm. Compared with Group BMPi, specimens in Group BMPm showed significantly greater early osteogenesis and evident osseointegration in the supra-alveolar level. Conclusion: BMP-2 loaded PLGA microspheres effectively promoted osteogenic potential of the gelatin/HA/Î²-TCP composite and facilitated supra-alveolar ridge augmentation inÂ vivo. Keywords: bone morphogenetic protein-2, bone regeneration, dental implant, tissue engineering, tissue scaffolds

Published in Journal of the Formosan Medical Association

ISSN: 0929-6646 (Print)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Medicine: Medicine (General)
Website: http://www.journals.elsevier.com/journal-of-the-formosan-medical-association/

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