Tracheal Repair with Human Umbilical Cord Mesenchymal Stem Cells Differentiated in Chondrocytes Grown on an Acellular Amniotic Membrane: A Pre-Clinical Approach
Paulo Ricardo Baggio Simeoni,
Rossana Baggio Simeoni,
Paulo André Bispo Machado Júnior,
Meila Bastos de Almeida,
Dilcele Silva Moreira Dziedzic,
Nádia Nascimento da Rosa,
Priscila E. Ferreira Stricker,
Anna Flávia Ribeiro dos Santos Miggiolaro,
Guilherme Naves,
Nelson Bergonse Neto,
Lucia de Noronha,
Julio Cesar Francisco,
Katherine Athayde Teixeira de Carvalho,
Luiz Cesar Guarita-Souza
Affiliations
Paulo Ricardo Baggio Simeoni
Experimental Laboratory of Institute of Biological and Health Sciences of Pontifical, Catholic University of Paraná (PUCPR), Street Imaculada Conceição, 1155, Curitiba 80215-901, Paraná, Brazil
Rossana Baggio Simeoni
Experimental Laboratory of Institute of Biological and Health Sciences of Pontifical, Catholic University of Paraná (PUCPR), Street Imaculada Conceição, 1155, Curitiba 80215-901, Paraná, Brazil
Paulo André Bispo Machado Júnior
Experimental Laboratory of Institute of Biological and Health Sciences of Pontifical, Catholic University of Paraná (PUCPR), Street Imaculada Conceição, 1155, Curitiba 80215-901, Paraná, Brazil
Meila Bastos de Almeida
Department of Veterinary Medicine, Universidade Federal do Paraná (UFPR), Rua XV de Novembro, 1299, Curitiba 80060-000, Paraná, Brazil
Dilcele Silva Moreira Dziedzic
Advanced Therapy and Cellular Biotechnology in Regenerative Medicine Research Group, Pelé Pequeno Príncipe Research Institute & Pequeno Príncipe Faculties (FPP) Ave., Silva Jardim, 1632, Curitiba 80240-020, Paraná, Brazil
Nádia Nascimento da Rosa
Advanced Therapy and Cellular Biotechnology in Regenerative Medicine Research Group, Pelé Pequeno Príncipe Research Institute & Pequeno Príncipe Faculties (FPP) Ave., Silva Jardim, 1632, Curitiba 80240-020, Paraná, Brazil
Priscila E. Ferreira Stricker
Advanced Therapy and Cellular Biotechnology in Regenerative Medicine Research Group, Pelé Pequeno Príncipe Research Institute & Pequeno Príncipe Faculties (FPP) Ave., Silva Jardim, 1632, Curitiba 80240-020, Paraná, Brazil
Anna Flávia Ribeiro dos Santos Miggiolaro
Experimental Laboratory of Institute of Biological and Health Sciences of Pontifical, Catholic University of Paraná (PUCPR), Street Imaculada Conceição, 1155, Curitiba 80215-901, Paraná, Brazil
Guilherme Naves
Experimental Laboratory of Institute of Biological and Health Sciences of Pontifical, Catholic University of Paraná (PUCPR), Street Imaculada Conceição, 1155, Curitiba 80215-901, Paraná, Brazil
Nelson Bergonse Neto
Experimental Laboratory of Institute of Biological and Health Sciences of Pontifical, Catholic University of Paraná (PUCPR), Street Imaculada Conceição, 1155, Curitiba 80215-901, Paraná, Brazil
Lucia de Noronha
Experimental Laboratory of Institute of Biological and Health Sciences of Pontifical, Catholic University of Paraná (PUCPR), Street Imaculada Conceição, 1155, Curitiba 80215-901, Paraná, Brazil
Julio Cesar Francisco
Experimental Laboratory of Institute of Biological and Health Sciences of Pontifical, Catholic University of Paraná (PUCPR), Street Imaculada Conceição, 1155, Curitiba 80215-901, Paraná, Brazil
Katherine Athayde Teixeira de Carvalho
Advanced Therapy and Cellular Biotechnology in Regenerative Medicine Research Group, Pelé Pequeno Príncipe Research Institute & Pequeno Príncipe Faculties (FPP) Ave., Silva Jardim, 1632, Curitiba 80240-020, Paraná, Brazil
Luiz Cesar Guarita-Souza
Experimental Laboratory of Institute of Biological and Health Sciences of Pontifical, Catholic University of Paraná (PUCPR), Street Imaculada Conceição, 1155, Curitiba 80215-901, Paraná, Brazil
Acellular amniotic membrane (AM) has been studied, with promising results on the reconstruction of lesioned tissues, and has become an attractive approach for tracheal repair. This study aimed to evaluate the repair of the trachea with human umbilical cord mesenchymal stem cells (hucMSCs) differentiated in chondrocytes, grown on an experimental model. Tracheal defects were induced by surgical tracheostomy in 30 New Zealand rabbits, and the acellular amniotic membrane, with or without cells, was covering the defect. The hucMSCs were isolated and cultivated with chondrogenic differentiation over the culture of 14 days, and then grown on the AM. In this study, the AM was biocompatible and hucMSCs differentiated into chondrocytes. Our results demonstrated an important role for AM with cultured cells in the promotion of immature collagen, known to produce tissue regeneration. In addition, cartilaginous tissue was found at the tracheal defects, demonstrated by immunohistology results. This study suggests that this biomaterial implantation can be an effective future therapeutic alternative for patients with tracheal injury.
