Phytochemical constituents and biological activities of Salvia macrosiphon Boiss.
Majid Balaei-Kahnamoei,
Mahdieh Eftekhari,
Mohammad Reza Shams Ardekani,
Tahmineh Akbarzadeh,
Mina Saeedi,
Hossein Jamalifar,
Maliheh Safavi,
Sohrab Sam,
Naghmeh Zhalehjoo,
Mahnaz Khanavi
Affiliations
Majid Balaei-Kahnamoei
Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences
Mahdieh Eftekhari
Department of Pharmacognosy and Pharmaceutical Biotechnology,School of Pharmacy, Kermanshah University of Medical Sciences
Mohammad Reza Shams Ardekani
Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences
Tahmineh Akbarzadeh
Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences
Mina Saeedi
Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences
Hossein Jamalifar
Quality Control of Pharmaceuticals and Supplements Group, Pharmaceutical Quality Assurance Research Center, The Institute of Pharmaceutical Sciences (TIPS), Tehran University of Medical Sciences
Maliheh Safavi
Department of Biotechnology, Iranian Research Organization for Science and Technology
Sohrab Sam
Department of Biotechnology, Iranian Research Organization for Science and Technology
Naghmeh Zhalehjoo
Department of Biochemistry, Genetics, Nutrition and Medicine, Alborz University of Medical Sciences
Mahnaz Khanavi
Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences
Abstract Salvia macrosiphon Boiss. is an aromatic perennial herb belonging to the family Lamiaceae. Phytochemical studies and biological activities of this plant have been rarely documented in the literature. The current study aimed to investigate antibacterial and cytotoxic activity of different fractions of aerial parts of S. macrosiphon. Also, we tried to isolate and identify cytotoxic compounds from the plant. In this respect, the hydroalcoholic extract of the corresponding parts of the plant was fractionated into four fractions. Then, antibacterial and cytotoxic activity of each fraction were examined. It was found that the chloroform fraction had a good antibacterial activity against gram-positive and gram-negative bacteria. The most potent cytotoxicity was also obtained by the n-hexane fraction comparing with etoposide as the reference drug which was selected for the study and characterization of secondary metabolites. Accordingly, 13-epi manoyl oxide (1), 6α-hydroxy-13-epimanoyl oxide (2), 5-hydroxy-7,4'-dimethoxyflavone (3), and β-sitosterol (4) were isolated and evaluated for their cytotoxic activity. Among them, compound 1 revealed significant cytotoxicity against A549, MCF-7, and MDA-MB-231. It merits mentioning that it showed high selectivity index ratio regarding the low cytotoxic effects on Human Dermal Fibroblast which can be considered as a promising anticancer candidate.
