Nature Communications (Jan 2016)
Structure of the polyisoprenyl-phosphate glycosyltransferase GtrB and insights into the mechanism of catalysis
- Chiara Ardiccioni,
- Oliver B. Clarke,
- David Tomasek,
- Habon A. Issa,
- Desiree C. von Alpen,
- Heather L. Pond,
- Surajit Banerjee,
- Kanagalaghatta R. Rajashankar,
- Qun Liu,
- Ziqiang Guan,
- Chijun Li,
- Brian Kloss,
- Renato Bruni,
- Edda Kloppmann,
- Burkhard Rost,
- M. Chiara Manzini,
- Lawrence Shapiro,
- Filippo Mancia
Affiliations
- Chiara Ardiccioni
- Department of Physiology and Cellular Biophysics, Columbia University
- Oliver B. Clarke
- Department of Biochemistry and Molecular Biophysics, Columbia University
- David Tomasek
- Department of Physiology and Cellular Biophysics, Columbia University
- Habon A. Issa
- Department of Pharmacology and Physiology, George Washington University
- Desiree C. von Alpen
- Department of Pharmacology and Physiology, George Washington University
- Heather L. Pond
- Department of Pharmacology and Physiology, George Washington University
- Surajit Banerjee
- NE-CAT and Department of Chemistry and Chemical Biology, Cornell University, Argonne National Laboratory
- Kanagalaghatta R. Rajashankar
- NE-CAT and Department of Chemistry and Chemical Biology, Cornell University, Argonne National Laboratory
- Qun Liu
- New York Structural Biology Center, X4 Beamlines, Brookhaven National Laboratory
- Ziqiang Guan
- Department of Biochemistry, Duke University Medical Center
- Chijun Li
- Department of Biochemistry, Duke University Medical Center
- Brian Kloss
- New York Consortium on Membrane Protein Structure, New York Structural Biology Center
- Renato Bruni
- New York Consortium on Membrane Protein Structure, New York Structural Biology Center
- Edda Kloppmann
- Department of Informatics, Bioinformatics and Computational Biology
- Burkhard Rost
- Department of Informatics, Bioinformatics and Computational Biology
- M. Chiara Manzini
- Department of Pharmacology and Physiology, George Washington University
- Lawrence Shapiro
- Department of Biochemistry and Molecular Biophysics, Columbia University
- Filippo Mancia
- Department of Physiology and Cellular Biophysics, Columbia University
- DOI
- https://doi.org/10.1038/ncomms10175
- Journal volume & issue
-
Vol. 7,
no. 1
pp. 1 – 9
Abstract
Polyisoprenyl-glycosyltransferases (PI-GTs) catalyse the addition of sugar to lipid carriers, which is the first step in the production of sugar donors for glycosylation. Here Ardiccioni et al.present the structure of a bacterial PI-GT and propose a mechanistic basis for sugar transfer.
