Ipomea aquatica EXTRACT INHIBITS LIPOXYGENASE, HYALURONIDASE AND XANTHINE OXIDASE ACTIVITIES

Abstract

Ipomea aquatica, locally known as water spinach, is one of the most common vegetable consumed by Malaysian. I. aquatica exhibited several biological activities including free radical scavenging, anti-microbial and anti-proliferative. The present study aims to enhance current knowledge on biological properties of I. aquatica particularly on anti-inflammatory activity. Three enzymes that involve in inflammatory pathway were selected in this study including lipoxygenase, hyaluronidase and xanthine oxidase. I. aquatica was extracted in methanol and tested for lipoxygenase, hyaluronidase and xanthine oxidase at different concentrations using direct enzyme inhibition assay. The results showed that I. aquatica extract dose-dependently inhibited lipoxygenase, hyaluronidase and xanthine oxidase enzyme activity. Highest inhibition activity against lipoxygenase, hyaluronidase and xanthine oxidase were observed at a concentration of 1 mg/mL with inhibition of 87.18%, 95.36% and 78.38%, respectively. Our finding in this study enhance indicates potential anti-inflammatory activity of I. aquatica through inhibition of lipoxygenase, hyaluronidase and xanthine oxidase.

Keywords

• ipomea aquatica
• lipoxygenase
• hyaluronidase
• xanthine oxidase
• anti-inflammatory