Black Soldier Fly Larva Oil in Diets with Roughage to Concentrate Ratios on Fermentation Characteristics, Degradability, and Methane Generation

Abstract

Currently, the scarcity of high-quality, expensive animal feed is a primary factor driving up the cost of animal husbandry. As a result, most researchers have focused on improving the potential of using alternative feed resources derived from the black soldier fly larva. In particular, the utilization of oil from black fly larvae is a byproduct of the industry. The aim of this study was to investigate the influence of black soldier fly larva oils and the proportion of roughage-to-concentrate ratios on gas kinetics, rumen characteristics, degradability, and mitigate CH4 production by using in vitro gas production techniques. The in vitro investigation used a completely randomized design (CRD) with a 2 × 4 factorial arrangement. The level of R:C ratios (60:40 and 40:60) were factor A, while BSFO levels (0, 2, 4, and 6% of DM) were factor B. Under this investigation, the combined impact of R:C ratio and BSFO on the kinetics of gas and accumulative gas production was found to be significant (p 3-N) concentration were found to be impacted by the inclusion of BSFO levels at different R:C-ratios (p 4 in the rumen (p p > 0.05), whereas increasing the concentration of BSFO in concentrate at 6% reduced the DM degradability after 24 h of incubation (p p 4 emission, and preserved DM degradability. A R:C ratio of 40:60 could improve the total volatile fatty acids and digestibility. Moreover, the inclusion of 6% BSFO at different R:C ratios lowered the in vitro dry matter digestibility, in vitro organic matter digestibility, NH3-N, and protozoal populations.

Keywords

• black soldier fly larva
• fermentation
• volatile fatty acid
• protozoa
• methane