Kasmera (Dec 2017)
The effect of praziquantel in the angiogenic receptors, inflammation and inflammatory cytokines on the murine intestinal schistosomiasis
Abstract
The studies on the pathogenic role of the Schistosoma mansoni focus on the hepatic damage and the immune response of the final host, in constrast to the limited information regarding intestinal lesion. This work evaluates. Intestinal granulomatous inflammation was measured in histological sections the effect of praziquantel in the angiogenic receptors (FLK1 and FLT1), inflammation and inflammatory cytokines on the murine intestinal schistosomiasis stained with H&E; the detection of TNF-α, IL-10, TGF-β, VEGF and its receptors was carried out by immunohistochemistry and ELISA, in serum and intestine sections of BALB/c mice infected with S. mansoni at 8 (RI8S) and 20 (RI20S) weeks post-infection, and mice infected for 8 weeks and evaluated 15 days post-treatment with praziquantel 40 μg/(RPT). In RI8S small granulomas (11.304 μm2 average), without sharp or marked edges within the granuloma; and large granulomas (70.650 μm2) with defined borders without defined zones within the granuloma, In both groups of mice macrophages predominated and plasma cells were present. In RI20S, granulomas have an inner zone composed by abundant macrophages and few plasma cells, and external zone constituted only by macrophages (11.985 μm2). In RPT, granulomas were not observed, only a few inflammatory foci nearby the muscularis mucosae and the Lieberkuhn glands. Localization of immune molecules in the intestine was only positive in RPT for VEGF, its receptors, TGF-β and IL-10; the results show a discrete response over the granuloma size, cellularity and cytokine expression at intestinal level.
