Reference Gene Selection and Gene Expression Analysis during Gall Development of <i>Zizania latifolia</i>
Yipeng Li,
Huan Yi,
Qing Gu,
Zhaisheng Zheng,
Mingxing Zhu,
Xiaojun Zha,
Shangfa Zhang,
Mengfei Yang
Affiliations
Yipeng Li
Zhejiang Provincial Key Laboratory of Characteristic Aquatic Vegetable Breeding and Cultivation, Jinhua Academy of Agricultural Sciences, Jinhua 321051, China
Huan Yi
College of Life Sciences, Zhejiang Normal University, Jinhua 321001, China
Qing Gu
College of Life Sciences, Zhejiang Normal University, Jinhua 321001, China
Zhaisheng Zheng
Zhejiang Provincial Key Laboratory of Characteristic Aquatic Vegetable Breeding and Cultivation, Jinhua Academy of Agricultural Sciences, Jinhua 321051, China
Mingxing Zhu
Zhejiang Provincial Key Laboratory of Characteristic Aquatic Vegetable Breeding and Cultivation, Jinhua Academy of Agricultural Sciences, Jinhua 321051, China
Xiaojun Zha
College of Life Sciences, Zhejiang Normal University, Jinhua 321001, China
Shangfa Zhang
Zhejiang Provincial Key Laboratory of Characteristic Aquatic Vegetable Breeding and Cultivation, Jinhua Academy of Agricultural Sciences, Jinhua 321051, China
Mengfei Yang
Zhejiang Provincial Key Laboratory of Characteristic Aquatic Vegetable Breeding and Cultivation, Jinhua Academy of Agricultural Sciences, Jinhua 321051, China
The stem tips of Zizania latifolia at different development stages were used as research materials. The expression stability of nine candidate reference genes (ACT1, H2B, UBI, EF-1α, GAPDH, β-actin, 60S, SKIP and AQP) were evaluated using qRT-PCR. The data were analyzed with GeNorm and NormFinder software. Present results indicated that the expression of ACT1 was stable and that it could be used as the optimal reference gene for studying the development stage of gall formation. ACT1 was selected as the reference gene to verify the expression level of the correlative genes in the gall formation stage of Z. latifolia. Our results were consistent with the previous transcriptome sequencing results. This study revealed that ACT1 was the classic reference gene for the analysis of correlative genes in all of the gall development stages of Z. latifolia.
