Овощи России (Jul 2018)
REGENERATION OF WINTER GARLIC PLANTS (ALLIUM SATIVUM L.) IN VITRO FROM BULBILS
Abstract
Garlic is exclusively vegetatively propagated plant which damaged by numerous virus, bacterial, fungus and other phytopathogens. Under propagation of damaged plants phytopathogens are transferred to progeny that leads to decrease in productivity, loss of quality, storability and frequently to degeneration of cultivars. In this regard improvement of landing material is obligate stage of the modern technologies of garlic production. Experimentally reasonable technology of the obtaining revitalized landing material of winter garlic based on in vitro cultivation of air bulbils on MS medium containing BA in concentration of 2 mg/l and NAA – 1 mg/l, which followed by formation of sprouts and then plants with bulbs is presented in the article. It is established that application of graduated sterilization of air bulbils isolated from not revealed inflorescences with diameter up to 25 mm for introduction in vitro culture allows to obtain plants free of internal infections. One of important factors influencing effectiveness of induction of morphogenesis is the plant genotype. Cultivar Emperor was characterized by high morphogenetic potential in vitro culture which significantly surpassed cultivar Gladiator at all stages of cultivation. The share of vital explants of Emperor cultivar consisted of 74,2%, and Gladiator cultivar – 56,3%. The morphogenesis of air bulbils in vitro conditions depends on their age. The share of vital micro bulbs with leaves formed under cultivation of air bulbils within 21 days isolated on 7th day after of exit of inflorescences from leaf sockets in depending on cultivar consisted of 65,0% and 76,4%. Cultivation of air bulbils of more old age is followed by their 100% growth with formation of leaves and microbulbs. Adaptation of such plants to ex vitro conditions is followed by obtaining monoclove bulbs cultivation of which in the open ground conditions leaded to formation of polyclove bulbs.Cultivation of plants in vitro on the medium containing sucrose in concentration of 10% gives the chance to keep viability material within 5-6 months.
Keywords
