Frontiers in Immunology (Oct 2024)
Analysis of lipid uptake, storage, and fatty acid oxidation by group 2 innate lymphoid cells
- Audrey Roy-Dorval,
- Audrey Roy-Dorval,
- Audrey Roy-Dorval,
- Rebecca C. Deagle,
- Rebecca C. Deagle,
- Rebecca C. Deagle,
- Frederik Roth,
- Frederik Roth,
- Frederik Roth,
- Mathilde Raybaud,
- Mathilde Raybaud,
- Mathilde Raybaud,
- Nailya Ismailova,
- Nailya Ismailova,
- Nailya Ismailova,
- Sai Sakktee Krisna,
- Sai Sakktee Krisna,
- Sai Sakktee Krisna,
- Damon G. K. Aboud,
- Camille Stegen,
- Camille Stegen,
- Camille Stegen,
- Julien Leconte,
- Julien Leconte,
- Julien Leconte,
- Gabriel Berberi,
- Gabriel Berberi,
- Gabriel Berberi,
- Ademola Esomojumi,
- Ademola Esomojumi,
- Ademola Esomojumi,
- Jörg H. Fritz,
- Jörg H. Fritz,
- Jörg H. Fritz,
- Jörg H. Fritz
Affiliations
- Audrey Roy-Dorval
- Department of Microbiology and Immunology, McGill University, Montréal, QC, Canada
- Audrey Roy-Dorval
- McGill University Research Center on Complex Traits (MRCCT), McGill University, Montréal, QC, Canada
- Audrey Roy-Dorval
- Dahdaleh Institute of Genomic Medicine (DIgM), McGill University, Montréal, QC, Canada
- Rebecca C. Deagle
- Department of Microbiology and Immunology, McGill University, Montréal, QC, Canada
- Rebecca C. Deagle
- McGill University Research Center on Complex Traits (MRCCT), McGill University, Montréal, QC, Canada
- Rebecca C. Deagle
- Dahdaleh Institute of Genomic Medicine (DIgM), McGill University, Montréal, QC, Canada
- Frederik Roth
- Department of Microbiology and Immunology, McGill University, Montréal, QC, Canada
- Frederik Roth
- McGill University Research Center on Complex Traits (MRCCT), McGill University, Montréal, QC, Canada
- Frederik Roth
- Dahdaleh Institute of Genomic Medicine (DIgM), McGill University, Montréal, QC, Canada
- Mathilde Raybaud
- Department of Microbiology and Immunology, McGill University, Montréal, QC, Canada
- Mathilde Raybaud
- McGill University Research Center on Complex Traits (MRCCT), McGill University, Montréal, QC, Canada
- Mathilde Raybaud
- Dahdaleh Institute of Genomic Medicine (DIgM), McGill University, Montréal, QC, Canada
- Nailya Ismailova
- Department of Microbiology and Immunology, McGill University, Montréal, QC, Canada
- Nailya Ismailova
- McGill University Research Center on Complex Traits (MRCCT), McGill University, Montréal, QC, Canada
- Nailya Ismailova
- Dahdaleh Institute of Genomic Medicine (DIgM), McGill University, Montréal, QC, Canada
- Sai Sakktee Krisna
- McGill University Research Center on Complex Traits (MRCCT), McGill University, Montréal, QC, Canada
- Sai Sakktee Krisna
- Dahdaleh Institute of Genomic Medicine (DIgM), McGill University, Montréal, QC, Canada
- Sai Sakktee Krisna
- Department of Physiology, McGill University, Montréal, QC, Canada
- Damon G. K. Aboud
- Department of Chemical Engineering, McGill University, Montréal, QC, Canada
- Camille Stegen
- Department of Microbiology and Immunology, McGill University, Montréal, QC, Canada
- Camille Stegen
- McGill University Research Center on Complex Traits (MRCCT), McGill University, Montréal, QC, Canada
- Camille Stegen
- Dahdaleh Institute of Genomic Medicine (DIgM), McGill University, Montréal, QC, Canada
- Julien Leconte
- Department of Microbiology and Immunology, McGill University, Montréal, QC, Canada
- Julien Leconte
- McGill University Research Center on Complex Traits (MRCCT), McGill University, Montréal, QC, Canada
- Julien Leconte
- Dahdaleh Institute of Genomic Medicine (DIgM), McGill University, Montréal, QC, Canada
- Gabriel Berberi
- Department of Microbiology and Immunology, McGill University, Montréal, QC, Canada
- Gabriel Berberi
- McGill University Research Center on Complex Traits (MRCCT), McGill University, Montréal, QC, Canada
- Gabriel Berberi
- Dahdaleh Institute of Genomic Medicine (DIgM), McGill University, Montréal, QC, Canada
- Ademola Esomojumi
- Department of Microbiology and Immunology, McGill University, Montréal, QC, Canada
- Ademola Esomojumi
- McGill University Research Center on Complex Traits (MRCCT), McGill University, Montréal, QC, Canada
- Ademola Esomojumi
- Dahdaleh Institute of Genomic Medicine (DIgM), McGill University, Montréal, QC, Canada
- Jörg H. Fritz
- Department of Microbiology and Immunology, McGill University, Montréal, QC, Canada
- Jörg H. Fritz
- McGill University Research Center on Complex Traits (MRCCT), McGill University, Montréal, QC, Canada
- Jörg H. Fritz
- Dahdaleh Institute of Genomic Medicine (DIgM), McGill University, Montréal, QC, Canada
- Jörg H. Fritz
- Department of Physiology, McGill University, Montréal, QC, Canada
- DOI
- https://doi.org/10.3389/fimmu.2024.1493848
- Journal volume & issue
-
Vol. 15
Abstract
Group 2 Innate Lymphoid Cells (ILC2) are critical drivers of both innate and adaptive type 2 immune responses, known to orchestrate processes involved in tissue restoration and wound healing. In addition, ILC2 have been implicated in chronic inflammatory barrier disorders in type 2 immunopathologies such as allergic rhinitis and asthma. ILC2 in the context of allergen-driven airway inflammation have recently been shown to influence local and systemic metabolism, as well as being rich in lipid-storing organelles called lipid droplets. However, mechanisms of ILC2 lipid anabolism and catabolism remain largely unknown and the impact of these metabolic processes in regulating ILC2 phenotypes and effector functions has not been extensively characterized. ILC2 phenotypes and effector functions are shaped by their metabolic status, and determining the metabolic requirements of ILC2 is critical in understanding their role in type 2 immune responses and their associated pathophysiology. We detail here a novel experimental method of implementing flow cytometry for large scale analysis of fatty acid uptake, storage of neutral lipids, and fatty acid oxidation in primary murine ILC2 with complementary morphological analysis of lipid storage using confocal microscopy. By combining flow cytometry and confocal microscopy, we can identify the metabolic lipid requirements for ILC2 functions as well as characterize the phenotype of lipid storage in ILC2. Linking lipid metabolism pathways to ILC2 phenotypes and effector functions is critical for the assessment of novel pharmaceutical strategies to regulate ILC2 functions in type 2 immunopathologies.
Keywords
- group 2 innate lymphoid cells (ILC2)
- type 2 immunity
- immunometabolism
- fatty acid uptake
- lipid droplets
- fatty acid oxidation (FAO)
