Plasmatic Oxidative and Metabonomic Profile of Patients with Different Degrees of Biliary Acute Pancreatitis Severity
Pedro Silva-Vaz,
Ivana Jarak,
Luís Rato,
Pedro F. Oliveira,
Sara Morgado-Nunes,
Aida Paulino,
Miguel Castelo-Branco,
Maria Filomena Botelho,
José Guilherme Tralhão,
Marco G. Alves,
Ana Margarida Abrantes
Affiliations
Pedro Silva-Vaz
Health Sciences Research Centre, University of Beira Interior (CICS-UBI), 6200-506 Covilhã, Portugal
Ivana Jarak
Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Coimbra, 3000-548 Coimbra, Portugal
Luís Rato
Health School of the Polytechnic of Guarda, 6300-559 Guarda, Portugal
Pedro F. Oliveira
QOPNA & LAQV, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal
Sara Morgado-Nunes
Clinical Academic Centre of Beiras (CACB), 6200-506 Covilhã, Portugal
Aida Paulino
General Surgery Department, Hospital Amato Lusitano, Unidade Local de Saúde de Castelo Branco, 6000-085 Castelo Branco, Portugal
Miguel Castelo-Branco
Health Sciences Research Centre, University of Beira Interior (CICS-UBI), 6200-506 Covilhã, Portugal
Maria Filomena Botelho
Biophysics Institute, Faculty of Medicina, University of Coimbra, 3000-548 Coimbra, Portugal
José Guilherme Tralhão
Biophysics Institute, Faculty of Medicina, University of Coimbra, 3000-548 Coimbra, Portugal
Marco G. Alves
Department of Anatomy and Unit for Multidisciplinary Research in Biomedicine (UMIB), Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, 4050-313 Porto, Portugal
Ana Margarida Abrantes
Biophysics Institute, Faculty of Medicina, University of Coimbra, 3000-548 Coimbra, Portugal
Acute pancreatitis (AP) is an inflammatory process of the pancreas with variable involvement of the pancreatic and peripancreatic tissues and remote organ systems. The main goal of this study was to evaluate the inflammatory biomarkers, oxidative stress (OS), and plasma metabolome of patients with different degrees of biliary AP severity to improve its prognosis. Twenty-nine patients with biliary AP and 11 healthy controls were enrolled in this study. We analyzed several inflammatory biomarkers, multifactorial scores, reactive oxygen species (ROS), antioxidants defenses, and the plasma metabolome of biliary AP and healthy controls. Hepcidin (1.00), CRP (0.94), and SIRI (0.87) were the most accurate serological biomarkers of AP severity. OS played a pivotal role in the initial phase of AP, with significant changes in ROS and antioxidant defenses relating to AP severity. Phenylalanine (p p p < 0.01) showed significant changes in AP severity. The role of hepcidin and SIRI were confirmed as new prognostic biomarkers of biliary AP. OS appears to have a role in the onset and progression of the AP process. Overall, this study identified several metabolites that may predict the onset and progression of biliary AP severity, constituting the first metabonomic study in the field of biliary AP.
