Characterization of Cupriavidus eutrophus В-10646 Culture Synthesizing Polyhydroxyalkanoates Grown on Sugars And Lipidic Substrates

Abstract

Growth, polyhydroxyalkanoates (PHAs) accumulation and total fatty acid composition of the lipids of Cupriavidus eutrophus B-10646 were studied, using various carbon sources (fructose, glucose, oleic acid, sunflower seed oil). The best substrates for biomass production (7.8-8.6 g/l) and polymer synthesis (7.3-7.9 g/l) were sugars and oleic acid. Bacterial cells grown on sugars and sunflower seed oil synthesized only homopolymer poly(3-hydroxybutyrate). 3-hydroxyvalerate (2.0-4.2 mol. %) was identified in polymer when Cupriavidus eutrophus used oleic acid as sole carbon source. Study of total fatty acid composition of lipids showed that major fatty acids were palmitic (16:0), palmitoleic (16:1ω7), and cis-vaccenic (18:1ω7) acids. When carbohydrate substrate was replaced by oleic acid or sunflower seed oil, the proportion of oleic acid in the total fatty acids increased considerably. In addition to that, the lipid fatty acids of bacterial cells grown on sunflower seed oil also contained linoleic acid, which is the major acid of sunflower seed oil

Keywords

• Cupriavidus eutrophus
• Cupriavidus eutrophus
• fatty
• acids
• polyhydroxyalkanoates
• oleic acid
• sunflower seed oil