Nature Communications (Apr 2022)
Genome-wide CRISPR screens identify GATA6 as a proviral host factor for SARS-CoV-2 via modulation of ACE2
- Ma’ayan Israeli,
- Yaara Finkel,
- Yfat Yahalom-Ronen,
- Nir Paran,
- Theodor Chitlaru,
- Ofir Israeli,
- Inbar Cohen-Gihon,
- Moshe Aftalion,
- Reut Falach,
- Shahar Rotem,
- Uri Elia,
- Ital Nemet,
- Limor Kliker,
- Michal Mandelboim,
- Adi Beth-Din,
- Tomer Israely,
- Ofer Cohen,
- Noam Stern-Ginossar,
- Adi Bercovich-Kinori
Affiliations
- Ma’ayan Israeli
- Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research
- Yaara Finkel
- Department of Molecular Genetics, Weizmann Institute of Science
- Yfat Yahalom-Ronen
- Department of Infectious Diseases, Israel Institute for Biological Research
- Nir Paran
- Department of Infectious Diseases, Israel Institute for Biological Research
- Theodor Chitlaru
- Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research
- Ofir Israeli
- Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research
- Inbar Cohen-Gihon
- Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research
- Moshe Aftalion
- Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research
- Reut Falach
- Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research
- Shahar Rotem
- Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research
- Uri Elia
- Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research
- Ital Nemet
- Central Virology Laboratory, Public Health Services, Ministry of Health and Sheba Medical Center, Tel Hashomer
- Limor Kliker
- Central Virology Laboratory, Public Health Services, Ministry of Health and Sheba Medical Center, Tel Hashomer
- Michal Mandelboim
- Central Virology Laboratory, Public Health Services, Ministry of Health and Sheba Medical Center, Tel Hashomer
- Adi Beth-Din
- Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research
- Tomer Israely
- Department of Infectious Diseases, Israel Institute for Biological Research
- Ofer Cohen
- Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research
- Noam Stern-Ginossar
- Department of Molecular Genetics, Weizmann Institute of Science
- Adi Bercovich-Kinori
- Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research
- DOI
- https://doi.org/10.1038/s41467-022-29896-z
- Journal volume & issue
-
Vol. 13,
no. 1
pp. 1 – 16
Abstract
Here, Israeli and Finkel et al. perform genome-wide CRISPR knockout screens to identify host factors required for the infection with SARS-CoV-2 and two additionally variants of concern, Alpha and Beta, unveiling shared and differential host pathways required by the variants, and demonstrate GATA6 is critical for SARS-CoV-2 viral entry through modulation of ACE2 expression.
