Detection of RNA-Dependent RNA Polymerase of Hubei Reo-Like Virus 7 by Next-Generation Sequencing in <i>Aedes aegypti</i> and <i>Culex quinquefasciatus</i> Mosquitoes from Brazil
Geovani de Oliveira Ribeiro,
Fred Julio Costa Monteiro,
Marlisson Octavio da S Rego,
Edcelha Soares D’Athaide Ribeiro,
Daniela Funayama de Castro,
Marcos Montani Caseiro,
Robson dos Santos Souza Marinho,
Shirley Vasconcelos Komninakis,
Steven S. Witkin,
Xutao Deng,
Eric Delwart,
Ester Cerdeira Sabino,
Antonio Charlys da Costa,
Élcio Leal
Affiliations
Geovani de Oliveira Ribeiro
Institute f Biological Sciences, Federal University of Pará, Av. Augusto Correa01, CEP 66075-000 Belém, Pará, Brazil
Fred Julio Costa Monteiro
Laboratório de Vetores, Superintendência de Vigilância em Saúde do Amapá, Rua Tancredo Neves, 1.118, CEP 68905-230 Macapá, AP, Brazil
Marlisson Octavio da S Rego
Laboratório de Vetores, Superintendência de Vigilância em Saúde do Amapá, Rua Tancredo Neves, 1.118, CEP 68905-230 Macapá, AP, Brazil
Edcelha Soares D’Athaide Ribeiro
Laboratório de Vetores, Superintendência de Vigilância em Saúde do Amapá, Rua Tancredo Neves, 1.118, CEP 68905-230 Macapá, AP, Brazil
Advancements in next-generation sequencing and bioinformatics have expanded our knowledge of the diversity of viruses (pathogens and non-pathogens) harbored by mosquitoes. Hubei reo-like virus 7 (HRLV 7) was recently detected by the virome analysis of fecal samples from migratory birds in Australia. We now report the detection of RNA-dependent RNA polymerase sequences of HRLV 7 in pools of Aedes aegypti and Culex quinquefasciatus mosquitoes species from the Brazilian Amazon forest. Phylogenetic inferences indicated that all HRLV 7 strains fall within the same independent clade. In addition, HRLV 7 shared a close ancestral lineage with the Dinovernavirus genus of the Reoviridae family. Our findings indicate that HRLV 7 is present in two species of mosquitoes.
