Co-Loading of Ascorbic Acid and Tocopherol in Eudragit-Nutriosomes to Counteract Intestinal Oxidative Stress

Maryam Rezvani; Maria  Letizia Manca; Carla Caddeo; Elvira Escribano-Ferrer; Claudia Carbone; José  Esteban Peris; Iris Usach; Octavio Diez-Sales; Anna  Maria Fadda; Maria Manconi

doi:10.3390/pharmaceutics11010013

Pharmaceutics (Jan 2019)

Co-Loading of Ascorbic Acid and Tocopherol in Eudragit-Nutriosomes to Counteract Intestinal Oxidative Stress

Maryam Rezvani,
Maria Letizia Manca,
Carla Caddeo,
Elvira Escribano-Ferrer,
Claudia Carbone,
José Esteban Peris,
Iris Usach,
Octavio Diez-Sales,
Anna Maria Fadda,
Maria Manconi

Affiliations

Maryam Rezvani: Department of Scienze della Vita e dell’Ambiente, University of Cagliari, via Ospedale 72, 09124 Cagliari, Italy
Maria Letizia Manca: Department of Scienze della Vita e dell’Ambiente, University of Cagliari, via Ospedale 72, 09124 Cagliari, Italy
Carla Caddeo: Department of Scienze della Vita e dell’Ambiente, University of Cagliari, via Ospedale 72, 09124 Cagliari, Italy
Elvira Escribano-Ferrer: Department of Pharmacy and Pharmaceutical Technology and Physical-Chemistry, Faculty of Pharmacy and Food Science, Institut of Nanoscience and Nanotechnology (IN2UB), University of Barcelona, Av. Joan XXIII s/n, 08028 Barcelona, Spain
Claudia Carbone: Department of Scienze del Farmaco, University of Catania, Viale A. Doria 6, 95125 Catania, Italy
José Esteban Peris: Department of Pharmacy, Pharmaceutical Technology and Parasitology, University of Valencia, Avda Vicente Andrés Estellés s/n, 46100 Burjassot, Valencia, Spain
Iris Usach: Department of Pharmacy, Pharmaceutical Technology and Parasitology, University of Valencia, Avda Vicente Andrés Estellés s/n, 46100 Burjassot, Valencia, Spain
Octavio Diez-Sales: Department of Pharmacy, Pharmaceutical Technology and Parasitology, University of Valencia, Avda Vicente Andrés Estellés s/n, 46100 Burjassot, Valencia, Spain
Anna Maria Fadda: Department of Scienze della Vita e dell’Ambiente, University of Cagliari, via Ospedale 72, 09124 Cagliari, Italy
Maria Manconi: Department of Scienze della Vita e dell’Ambiente, University of Cagliari, via Ospedale 72, 09124 Cagliari, Italy

DOI: https://doi.org/10.3390/pharmaceutics11010013
Journal volume & issue: Vol. 11, no. 1
p. 13

Abstract

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The present study aimed at developing a new vesicular formulation capable of promoting the protective effect of ascorbic acid and tocopherol against intestinal oxidative stress damage, and their efficacy in intestinal wound healing upon oral administration. A pH-dependent copolymer (Eudragit® L100), a water-soluble prebiotic fibre (Nutriose® FM06), a phospholipid mixture (Lipoid S75), and two natural antioxidants (ascorbic acid and tocopherol) were combined to fabricate eudragit-nutriosomes by a simple, solvent-free procedure. The vesicles were spherical and oligolamellar, with some multicompartment structures in Eudragit-nutriosomes, small in size (~100 nm), with highly negative zeta potential. The effect of Eudragit® and Nutriose® on the stability on storage and in simulated gastrointestinal fluids were confirmed by the Turbiscan® technology and in vitro studies, respectively. Eudragit-nutriosomes exhibited a protective effect against H2O2-induced oxidative stress, and a proliferative effect in Caco-2 cells, as they provided the closure of the scratched area after 96 h of incubation.

Published in Pharmaceutics

ISSN: 1999-4923 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Medicine: Pharmacy and materia medica
Website: http://www.mdpi.com/journal/pharmaceutics/

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