Co-Loading of Ascorbic Acid and Tocopherol in Eudragit-Nutriosomes to Counteract Intestinal Oxidative Stress
Maryam Rezvani,
Maria Letizia Manca,
Carla Caddeo,
Elvira Escribano-Ferrer,
Claudia Carbone,
José Esteban Peris,
Iris Usach,
Octavio Diez-Sales,
Anna Maria Fadda,
Maria Manconi
Affiliations
Maryam Rezvani
Department of Scienze della Vita e dell’Ambiente, University of Cagliari, via Ospedale 72, 09124 Cagliari, Italy
Maria Letizia Manca
Department of Scienze della Vita e dell’Ambiente, University of Cagliari, via Ospedale 72, 09124 Cagliari, Italy
Carla Caddeo
Department of Scienze della Vita e dell’Ambiente, University of Cagliari, via Ospedale 72, 09124 Cagliari, Italy
Elvira Escribano-Ferrer
Department of Pharmacy and Pharmaceutical Technology and Physical-Chemistry, Faculty of Pharmacy and Food Science, Institut of Nanoscience and Nanotechnology (IN2UB), University of Barcelona, Av. Joan XXIII s/n, 08028 Barcelona, Spain
Claudia Carbone
Department of Scienze del Farmaco, University of Catania, Viale A. Doria 6, 95125 Catania, Italy
José Esteban Peris
Department of Pharmacy, Pharmaceutical Technology and Parasitology, University of Valencia, Avda Vicente Andrés Estellés s/n, 46100 Burjassot, Valencia, Spain
Iris Usach
Department of Pharmacy, Pharmaceutical Technology and Parasitology, University of Valencia, Avda Vicente Andrés Estellés s/n, 46100 Burjassot, Valencia, Spain
Octavio Diez-Sales
Department of Pharmacy, Pharmaceutical Technology and Parasitology, University of Valencia, Avda Vicente Andrés Estellés s/n, 46100 Burjassot, Valencia, Spain
Anna Maria Fadda
Department of Scienze della Vita e dell’Ambiente, University of Cagliari, via Ospedale 72, 09124 Cagliari, Italy
Maria Manconi
Department of Scienze della Vita e dell’Ambiente, University of Cagliari, via Ospedale 72, 09124 Cagliari, Italy
The present study aimed at developing a new vesicular formulation capable of promoting the protective effect of ascorbic acid and tocopherol against intestinal oxidative stress damage, and their efficacy in intestinal wound healing upon oral administration. A pH-dependent copolymer (Eudragit® L100), a water-soluble prebiotic fibre (Nutriose® FM06), a phospholipid mixture (Lipoid S75), and two natural antioxidants (ascorbic acid and tocopherol) were combined to fabricate eudragit-nutriosomes by a simple, solvent-free procedure. The vesicles were spherical and oligolamellar, with some multicompartment structures in Eudragit-nutriosomes, small in size (~100 nm), with highly negative zeta potential. The effect of Eudragit® and Nutriose® on the stability on storage and in simulated gastrointestinal fluids were confirmed by the Turbiscan® technology and in vitro studies, respectively. Eudragit-nutriosomes exhibited a protective effect against H2O2-induced oxidative stress, and a proliferative effect in Caco-2 cells, as they provided the closure of the scratched area after 96 h of incubation.
