Bioinformatics Analysis of Linear B-cell Viscumin Toxin Epitope With Potential Use in Molecularly Imprinted Polymer Biosensors

Abstract

Background: There are many diseases around the world that threaten human health and its related hygienic issues. Cancer is among the conditions mentioned above that cause many problems for health sectors worldwide. Methods: The present research analyzed the linear B-cell epitope of viscumin from European mistletoe using bioinformatics tools. We also provided references for the fast detection of biological agents. Several important tools, such as Protparam, NCBI, PDB, T-coffee, BCpred, Bptope, Ellipro, and Cn3D were used to predict the viscumin linear epitope and its physical and chemical properties. Results: The 9-mer epitope found as QQTTGEEYF embedded in the A-chain of protein by the least sequence homology with other homologous rivals. Its molecular weight, theoretical isoelectric point, and the total number of negatively charged residues were 1102.1, 3.79, and 2, respectively. Conclusion: Using different databases and establishing the accuracy level of ˃50% for linear B-cell epitope prediction, the selected epitope passed the related criteria and was introduced as a new linear epitope as a potential biological element in biosensors for cancer (viscumin) fast therapeutic detection.

Keywords

• bioinformatics
• biosensors
• viscumin
• mistletoe lectin 1