Enhanced Plasmonic Biosensor Utilizing Paired Antibody and Label-Free Fe3O4 Nanoparticles for Highly Sensitive and Selective Detection of Parkinson’s α-Synuclein in Serum

Samuel Husin Surya Mandala; Tai-Jan Liu; Chiung-Mei Chen; Kuo-Kang Liu; Mochamad Januar; Ying-Feng Chang; Chao-Sung Lai; Kuo-Hsuan Chang; Kou-Chen Liu

doi:10.3390/bios11100402

Biosensors (Oct 2021)

Enhanced Plasmonic Biosensor Utilizing Paired Antibody and Label-Free Fe3O4 Nanoparticles for Highly Sensitive and Selective Detection of Parkinson’s α-Synuclein in Serum

Samuel Husin Surya Mandala,
Tai-Jan Liu,
Chiung-Mei Chen,
Kuo-Kang Liu,
Mochamad Januar,
Ying-Feng Chang,
Chao-Sung Lai,
Kuo-Hsuan Chang,
Kou-Chen Liu

Affiliations

Samuel Husin Surya Mandala: Department of Electronic Engineering, Chang Gung University, Taoyuan 33302, Taiwan
Tai-Jan Liu: Department of Traditional Chinese Medicine, Chang Gung Memorial Hospital, Keelung 204, Taiwan
Chiung-Mei Chen: Department of Neurology, Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Taoyuan 33302, Taiwan
Kuo-Kang Liu: School of Engineering, University of Warwick, Coventry CV4 7AL, UK
Mochamad Januar: Department of Electronic Engineering, Chang Gung University, Taoyuan 33302, Taiwan
Ying-Feng Chang: Artificial Intelligence Research Center, Chang Gung University, Taoyuan 33302, Taiwan
Chao-Sung Lai: Department of Electronic Engineering, Chang Gung University, Taoyuan 33302, Taiwan
Kuo-Hsuan Chang: Department of Neurology, Chang Gung Memorial Hospital Linkou Medical Center and College of Medicine, Chang Gung University, Linkou 33305, Taiwan
Kou-Chen Liu: Department of Electronic Engineering, Chang Gung University, Taoyuan 33302, Taiwan

DOI: https://doi.org/10.3390/bios11100402
Journal volume & issue: Vol. 11, no. 10
p. 402

Abstract

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Parkinson’s disease (PD) is an acute and progressive neurodegenerative disorder, and diagnosis of the disease at its earliest stage is of paramount importance to improve the life expectancy of patients. α-Synuclein (α-syn) is a potential biomarker for the early diagnosis of PD, and there is a great need to develop a biosensing platform that precisely detects α-syn in human body fluids. Herein, we developed a surface plasmon resonance (SPR) biosensor based on the label-free iron oxide nanoparticles (Fe3O4 NPs) and paired antibody for the highly sensitive and selective detection of α-syn in serum samples. The sensitivity of the SPR platform is enhanced significantly by directly depositing Fe3O4 NPs on the Au surface at a high density to increase the decay length of the evanescent field on the Au film. Moreover, the utilization of rabbit-type monoclonal antibody (α-syn-RmAb) immobilized on Au films allows the SPR platform to have a high affinity-selectivity binding performance compared to mouse-type monoclonal antibodies as a common bioreceptor for capturing α-syn molecules. As a result, the current platform has a detection limit of 5.6 fg/mL, which is 20,000-fold lower than that of commercial ELISA. The improved sensor chip can also be easily regenerated to repeat the α-syn measurement with the same sensitivity. Furthermore, the SPR sensor was applied to the direct analysis of α-syn in serum samples. By using a format of paired α-syn-RmAb, the SPR sensor provides a recovery rate in the range from 94.5% to 104.3% to detect the α-syn in diluted serum samples precisely. This work demonstrates a highly sensitive and selective quantification approach to detect α-syn in human biofluids and paves the way for the future development in the early diagnosis of PD.

Published in Biosensors

ISSN: 2079-6374 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Technology: Chemical technology: Biotechnology
Website: http://www.mdpi.com/journal/biosensors

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