PRODUCTION AND UTILIZATION OF CELLULASE FROM TRICHODERMA VIRIDE

Abstract

 An appropriate preservation technology for forage such as silage needs to be developed in order to overcome the shortage of tropical forage during the dry season. A good quality of silage is obtained by decreasing the fibre contents (Neutral Detergent Fibre, Acid Detergent Fibre and lignin). The research was conducted in two stages: 1). Production and activity test of crude enzymes from Trichoderma viride and 2). Comparative test using crude enzymes from Trichoderma viride strain QM 9414 (CETV) and commercial cellulase (Cellulase “Onozuka R-10”, heidelberg) on rice straw silage. The treatments were arranged as follows : P0=Untreated rice straw silage, P1=Treated with commercial cellulase at 10 IU kg-1 , P2= Treated with CETV at 3.8 IU kg-1 , P3=Treated with CETV at 7.6 IU kg-1  and P4= Treated with CETV at 11.4 IU kg-1  of fresh rice straw. All treatments were enriched with 5% molasses. The result of the research indicated that: 1). The activity of crude enzymes from Trichoderma viride strain QM 9414 (CETV) was 1.52 IUml -1 . 2). The addition of both enzymes did not decrease dry matter of rice straw silage; however, organic matter significantly decreased (p