Molecules (Nov 2019)

Efficient Enzymatic Hydrolysis of Biomass Hemicellulose in the Absence of Bulk Water

  • Shaghayegh Ostadjoo,
  • Fabien Hammerer,
  • Karolin Dietrich,
  • Marie-Josée Dumont,
  • Tomislav Friščić,
  • Karine Auclair

DOI
https://doi.org/10.3390/molecules24234206
Journal volume & issue
Vol. 24, no. 23
p. 4206

Abstract

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Current enzymatic methods for hemicellulosic biomass depolymerization are solution-based, typically require a harsh chemical pre-treatment of the material and large volumes of water, yet lack in efficiency. In our study, xylanase (E.C. 3.2.1.8) from Thermomyces lanuginosus is used to hydrolyze xylans from different sources. We report an innovative enzymatic process which avoids the use of bulk aqueous, organic or inorganic solvent, and enables hydrolysis of hemicellulose directly from chemically untreated biomass, to low-weight, soluble oligoxylosaccharides in >70% yields.

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