COMPARATIVE EVALUATION OF MICROBIOCENOSES OF THE CERVICAL CANAL DISCHARGE AND THE EJACULATE IN MARRIED COUPLES

Abstract

The study objective is to perform comparative evaluation of the microbiocenoses of the cervical canal discharge and the ejaculate in married couples using standard bacteriological culture and real-time polymerase chain reaction (RT-PCR) test systems Androflor for men and Femoflor for women.Materials and methods. Retrospective analysis of medical histories of 154 married couples seeking medical help in the preconception period was conducted. All patients were divided into 2 groups: in 121 married couples, standard bacteriological cultures of the cervical canal discharge and the ejaculate were evaluated; in 33 couples, analysis of the cervical canal discharge and the ejaculate was performed using RTPCR test systems Androflor and Femoflor.Results. Monoinfection was significantly more frequent in the ejaculate than in the cervical canal discharge (p <0.05). In women, cultures were significantly more frequently identified as sterile compared to men (p = 0.01). According to the results of standard bacteriological study, in most observations microbiocenoses of the cervical canal discharge and the ejaculate of married couples didn’t match fully in microorganism composition (69.4 %) and matched exactly only in 18.2 % of cases. Evaluation using RT-PRC showed different microorganisms in the microbiocenoses of the ejaculate and the cervical canal discharge in 48 % of observations.Conclusion. The study demonstrates the uniqueness of urogenital microbiocenoses and attests to the necessity of examination of both partners at the stage of preconception preparation and unacceptability of prescribing the same treatment to both partners.

Keywords

• ejaculate
• cervical canal
• microbiocenosis
• married couple
• bacteriological culture
• real-time polymerase chain reaction
• аndroflor
• femoflor