Optimization of Bioprocess Extraction of <i>Poria cocos</i> Polysaccharide (PCP) with <i>Aspergillus niger</i> β-Glucanase and the Evaluation of PCP Antioxidant Property
Peng Wu,
Hongyuan Tan,
Jianfeng Zhan,
Weixin Wang,
Ting Hu,
Shiming Li
Affiliations
Peng Wu
Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization, Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountain, Hubei Zhongke Industrial Technology Research Institute, Huanggang Normal University, Huanggang 438000, China
Hongyuan Tan
Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization, Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountain, Hubei Zhongke Industrial Technology Research Institute, Huanggang Normal University, Huanggang 438000, China
Jianfeng Zhan
Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization, Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountain, Hubei Zhongke Industrial Technology Research Institute, Huanggang Normal University, Huanggang 438000, China
Weixin Wang
Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization, Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountain, Hubei Zhongke Industrial Technology Research Institute, Huanggang Normal University, Huanggang 438000, China
Ting Hu
Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization, Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountain, Hubei Zhongke Industrial Technology Research Institute, Huanggang Normal University, Huanggang 438000, China
Shiming Li
Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization, Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountain, Hubei Zhongke Industrial Technology Research Institute, Huanggang Normal University, Huanggang 438000, China
Poria cocos mushroom is widely used as a food and an herb in East Asian and other countries due to its high nutritional value. Research has demonstrated that Poria cocos polysaccharides (PCP) are the major bioactives and possess antioxidation, anti-inflammation, immunoregulation, and other health promoting properties. However, the efficient preparation of PCP has been a challenge, particularly in large scale for industry. Herein, we investigated the biotransformation of PCP from Poria cocos, catalyzed by β-glucanase from Aspergillus niger and focused on optimizing the most four influencing parameters: Temperature, time, pH, and enzyme dosage in this study. After numerous optimizations with the assistance of response surface optimization methodology, we have established that the optimal conditions for the biotransformation PCP preparation were as following: Enzymolysis temperature 60 °C, time 120 min, pH 5.0 and enzyme dose 20 mL. Under these conditions, the extraction yield of PCP reached as high as 12.8%. In addition, the antioxidant activities of PCP were evaluated by reducing power assay and 1,1-diphenyl-2-picryl-hydrazyl, superoxide anion, and hydroxyl radicals scavenging assays. Resulting data showed that PCP presented outstanding antioxidant capacity. Thus, these findings indicate that PCP could be produced as a natural antioxidant for further development.
