Semi-stable production of bovine IL-4 and GM-CSF in the mammalian episomal expression system

Abstract

Granulocyte-macrophage colony stimulating factor (GM-CSF) and interleukin-4 (IL-4) are cytokines widely used in ex vivo monocyte differentiation experiments, vaccine formulations and disease treatment. The aim of this study was to produce recombinant bovine GM-CSF and IL-4 in an episomal expression system that conserves the postransductional modification of the native proteins and to use the products to differentiate bovine monocytes into dendritic cells.

Keywords

• bovine gm-csf
• bovine il-4
• dendritic cells
• macrophages
• mammalian episomal expression